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谢晓亮
研究员

  • E-mail:xie#chemistry.harvard.edu
  • 办公电话:+86-10-6274.4061 +1-617-496.9925
    办公地点:北京大学综合科研楼生物动态光学成像中心301室
    邮件地址:北京市海淀区颐和园路5号北京大学综合科研楼生物动态光学成像中心301室,100871
    研究组主页:http://xielab.pku.edu.cn
  • 理学博士,美国加州大学圣地亚哥分校,1990,化学
    理学学士,北京大学,1984,化学

    工作经历
  • 2016.8-现在,北大未来基因诊断高精尖创新中心主任

    2010-现在,北京大学生物动态光学成像中心主任

    2010-现在,北京大学化学与分子工程学院,客座教授

    2009-现在,北京大学生命科学学院,长江特聘讲座教授,

    2009-现在,哈佛大学化学与化学生物学系,Mallinckrodt讲席教授

    1999-2008,哈佛大学化学与化学生物学系,化学教授

    1992-1998,美国太平洋西北国家实验室(NPPL),环境分子科学实验室,Senior Research Scientist to Chief Scientist

    1990-1992,美国芝加哥大学博士后

    主要研究方向
  • 单分子酶学、 基因表达、染色体结构,单细胞基因组学,非线性光学成像技术及其生物与医学应用
    获奖及荣誉
  • 2016,美国医学科学院院士

    2016,华人生物学家协会吴瑞奖

    2015,中国生物物理学会贝时璋生物物理奖

    2015,美国Albany 医学奖

    2015,美国化学会Peter Debye 医学奖

    2014,美国光学会会士

    2014,德国洪堡学者

    2013,美国NIH Director's Pioneer Award

    2013,美国光学学会及应用光谱学会Ellis R. Lippincott

    2012,美国化学会Rochester分部Harrison Howe

    2012,美国微生物学会会士

    2012,美国生物物理学会Founders

    2011,美国科学院院士

    2010,中国化学会荣誉会士

    2009,美国能源部劳伦斯奖,化学类

    2008,美国物理学会会士

    2008,美国艺术与科学学院,院士

    2008Berthold Leibinger Zukunftspreis激光奖

    2007Willis E.Lamb激光科学与量子光学奖

    2006,美国科学促进会(AAAS)会士

    2006,美国生物物理学会会士

    2004,美国NIH Director's Pioneer Award

    2003,以色列Raymond and Beverly Sackler 物理科学奖

    1996Coblentz协会,Coblentz


    代表性论文及论著

  • Single Cell Genomics

    • Xu, Juanjuan; Fang, Rui; Chen, Li; Chen, Daozhen; Xiao, Jian-Ping; Yang, Weimin; Wang, Honghua; Song, Xiaoqing; Ma, Ting; Bo, Shiping; Shi, Chong; Ren, Jun; Huang, Lei; Cai, Li-Yi; Yao, Bing; Xie, X Sunney; Lu, Sijia "Noninvasive chromosome screening of human embryos by genome sequencing of embryo culture medium for in vitro fertilization," Proc Natl Acad Sci USA 113(42), 11907-11912 (2016).

    • Yan, Liying; Huang, Lei; Xu, Liya; Huang, Jin; Ma, Fei; Zhu, Xiaohui; Tang, Yaqiong; Liu, Mingshan; Lian, Ying; Liu, Ping; Li, Rong; Lu, Sijia; Tang, Fuchou; Qiao, Jie; Xie, X Sunney "Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses," Proc Natl Acad Sci USA, 112(52), 15964-15969 (2015).

    • Hou, Yu; Fan, Wei; Yan, Liying; Li, Rong; Lian, Ying; Huang, Jin; Li, Jinsen; Xu, Liya; Tang, Fuchou; Xie, X. Sunney; Qiao, Jie. "Genome Analyses of Single Human Oocytes," Cell 155, 1492-1506 (2013)

    • Ni, Xiaohui; Zhuo, Minglei; Su, Zhe; Duan, Jianchun; Gao, Yan; Wang, Zhijie; Zong, Chenghang; Bai, Hua; Chapman, Alec R.; Zhao, Jun; Xu, Liya; An, Tongtong; Ma, Qi; Wang, Yuyan; Wu, Meina; Sun, Yu; Wang, Shuhang; Li, Zhenxiang; Yang, Xiaodan; Yong, Jun; Su, Xiao-Dong; Lu, Youyong; Bai, Fan; Xie, X. Sunney; Wang, Jie. "Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients," PNAS 110, 21083-21088 (2013)

    • Zong, Chenghang; Lu, Sijia; Chapman, Alec R.; Xie, X. Sunney."Genome-Wide Detection of Single-Nucleotide and Copy-Number Variations of a Single Human Cell" Science 338, 1622-1626 (2012).

    • Lu, Sijia; Zong, Chenghang; Fan, Wei; Yang, Mingyu; Li, Jinsen; Chapman, Alec R.; Zhu, Ping; Hu, Xuesong; Xu, Liya; Bai, Fan; Qiao, Jie; Tang, Fuchou; Li, Ruiqiang; Xie, X. Sunney. "Probing Meiotic Recombination and Aneuploidy of Single Sperm Cells by Whole-Genome Sequencing" Science 338, 1627-1630 (2012).

    • Shiroguchi, Katsuyuki; Jia, Tony Z.; Sims, Peter A.; Xie, X. Sunney."Digital RNA Sequencing Minimizes Sequence-dependent Bias and Amplification Noise with Optimized Single-molecule Barcodes," PNAS 109, 1347-1352 (2012).

    • Sims, Peter A.; Greenleaf, William J.; Duan, Haifeng; Xie, X. Sunney. "Fluorogenic DNA Sequencing in PDMS Microreactors," Nat Methods 8, 575-580 (2011).


    Gene Expression and Regulation - Live Cell Single Molecule Studies

    • Chong, Shasha; Chen, Chongyi; Ge, Hao; Xie, X. Sunney. "Mechanism of Transcriptional Bursting in Bacteria," Cell 158, 314-326 DOI:10.1016/j.cell.2014.05.038 (2014)

    • Taniguchi, Yuichi; Choi, Paul J.; Li, Gene-Wei; Chen, Huiyi; Babu, Mohan; Hearn, Jeremy; Emili, Andrew; Xie, X. Sunney. "Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells," Science 329, 533-538 (2010).

    • Choi, Paul J.; Cai, Long; Frieda, Kirsten; Xie, X. Sunney. "A Stochastic Single-Molecule Event Triggers Phenotype Switching of a Bacterial Cell," Science 322, 442-446 (2008).

    • Elf, Johan; Li, Gene-Wei; Xie, X. Sunney. "Probing Transcription Factor Dynamics at the Single-Molecule Level in a Living Cell," Science 316, 1191-1194 (2007).

    • Yu, Ji; Xiao, Jie; Ren, Xiaojia; Lao, Kaiqin; Xie, X. Sunney. "Probing Gene Expression in Live Cells, One Protein Molecule at a Time," Science 311, 1600-1603 (2006).

    • Cai, Long; Friedman, Nir; Xie, X. Sunney. "Stochastic Protein Expression in Individual Cells at the Single Molecule Level," Nature 440, 358-362 (2006).


    Single Molecule Enzymology

    • Kim, Sangjin; Brostr?mer, Erik; Xing, Dong; Jin, Jianshi; Chong, Shasha; Ge, Hao; Wang, Siyuan; Gu, Chan; Yang, Lijiang; Qin-gao, Yi; Su, Xiao-dong; Sun, Yujie; Xie, X. Sunney. "Probing Allostery Through DNA" Science 339, 816-819 (2013).

    • Blainey, Paul C.; Luo, Guobin; Ko9u, S.C.; Mangel, Walter F.; Verdine, Gregory L.; Bagchi, Biman; Xie, X. Sunney. "Nonspecifically Bound Proteins Spin While Diffusing Along DNA," Nat Struct Mol Biol 16, 1224-1229 (2009).

    • Luo, Guobin; Wang, Mina; Konigsberg, William H.; Xie, X. Sunney. "Single-Molecule and Ensemble Fluorescence Assays for a Functionally Important Conformational Change in T7 DNA Polymerase," Proc Natl Acad Sci USA 104, 12610-12615 (2007).

    • English, Brian P.; Min, Wei; van Oijen, Antoine M.; Lee, Kang Taek; Luo, Guobin; Sun, Hongye; Cherayil, Binny J.; Kou, S.C.; Xie, X. Sunney. "Ever-Fluctuating Single Enzyme Molecules: Michaelis-Menten Equation Revisited," Nat Chem Bio 2, 87-94 (2006).

    • Min, Wei; Luo, Guobin; Cherayil, Binny J.; Kou, S. C.; Xie, X. Sunney. "Observation of a Power-Law Memory Kernel for Fluctuations within a Single Protein Molecule," Phys Rev Lett 94, 198302(1-4) (2005).

    • van Oijen, Atoine M. ; Blainey, Paul C. ; Crampton, Donald J. ; Richardson, Charles C. ; Ellenberger, Tom; Xie, X. Sunney. "Single-Molecule Kinetics of l Exonuclease Reveal Base Dependence and Dynamic Disorder," Science 301, 1235-1238 (2003).

    • Yang, Haw; Luo, Guobin; Karnchanaphanurach, Pallop; Louie, Tai-Man; Rech, Ivan; Cova, Sergio; Xun, Luying; Xie, X. Sunney. "Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer," Science 302, 262-266 (2003).  Lu, H. Peter; Xun, Luying; Xie, X. Sunney. "Single-Molecule Enzymatic Dynamics," Science 282, 1877-1882 (1998).


    Single Molecule Spectroscopy and Imaging

    • Zhou, Yuexing; Wang Ping; Tian, Feng, Gao, Ge; Huang, Lei; Wei, Wensheng; and Xie, X. Sunney “Painting A Specific Chromosome with CRISPR/Cas9 for Live-cell Imaging”. Cell Res.  2017 (in press).

    • Chong, Shasha; Min, Wei; Xie, X. Sunney. "Ground-State Depletion Microscopy:Detection Sensitivity of Single-Molecule Optical Absorption at Room Temperature,"J Phys Chem Lett 1, 3316-3322 (2010).

    • Min, Wei; Lu, Sijia; Chong, Shasha; Roy, Rahul; Holtom, Gary R.; Xie, X. Sunney. "Imaging Chromophores with Undetectable Fluorescence by Stimulated Emission Microscopy," Nature 461, 1105-1109 (2009).

    • Sánchez, Erik J.; Novotny, Lukas; Xie, X. Sunney. "Near-field Fluorescence Microscopy Based on Two-photon Excitation with Metal Tips," Phys Rev Lett 82, 4014-4017 (1999).

    •  Lu, H. Peter; Xie, X. Sunney. "Single-Molecule Spectral Fluctuations at Room Temperature," Nature 385, 143-146 (1997).

    • Sánchez, Erik J.; Novotny, Lukas; Holtom, G.R.; Xie, X. Sunney. "Room-Temperature Fluorescence Imaging and Spectroscopy of Single Molecules by Two-Photon Excitation," J Phys Chem A 101, 7019-7023 (1997).

    • Xie, X. Sunney; Dunn, Robert C. "Probing Single Molecule Dynamics," Science 265, 361-364 (1994).、

    Coherent Raman Microscopy

    • Ji, Minbiao; Orringer, Daniel A.; Freudiger, Christian W.; Ramkissoon, Shakti; Liu, Xiaohui; Lau, Darryl; Golby, Alexandra J.; Norton, Isaiah; Hayashi, Marika; Agar, Nathalie Y. R.; Young, Geoffrey S.; Spino, Cathie; Santagata, Sandro; Camelo-Piragua, Sandra; Ligon, Keith L.; Sagher, Oren; Xie, X. Sunney. "Rapid, Label-Free Detection of Brain Tumors with Stimulated Raman Scattering Microscopy," Science Translational Medicine 5, 201ra119 DOI: 10.1126/scitranslmed.3005954 (2013).

    •  Wang, Meng C.; Min, Wei; Freudiger, Christian W.; Ruvkun, Gary; Xie, X. Sunney. "RNAi Screening for Fat Regulatory Genes with SRS Microscopy," Nat Methods 8, 135-138 (2011).

    •  Saar, Brian G.; Freudiger, Christian W.; Reichman, Jay; Stanley, C. Michael; Holtom, Gary R.; Xie, X. Sunney. "Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering," Science 330, 1368-1370 (2010).

    •  Freudiger, Christian W.; Min, Wei; Saar, Brian G.; Lu, Sijia; Holtom, Gary R.; He, Chengwei; Tsai, Jason C.; Kang, Jing X.; Xie, X. Sunney. "Label-Free Biomedical Imaging with High sensitivity by Stimulated Raman Scattering Microscopy," Science 322, 1857-1860 (2008).

    • Evans, Conor L.; Potma, Eric O.; Puoris'haag, Mehron; C?té, Daniel; Lin, Charles P.; Xie, X. Sunney. "Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy" Proc Nat. Aca Sci 102, 16807-16812 (2005).

    • Zumbusch, Andreas; Holtom, Gary R.; Xie, X. Sunney. "Vibrational Microscopy Using Coherent Anti-Stokes Raman Scattering," Phys Rev Lett 82, 4142-4145 (1999).