Haifeng Duan
Research Associate Professor

  • E-mail:hfduan@yahoo.com
  • Tel:+86-10-6275.8404
    Office:Rm. N442, School of Life Sciences, Peking University
    Mailing Address:Rm. N442, School of Life Sciences, Peking University,100871
  • Ph. D. Nankai University,Tianjin, P. R. China, 2006, Chemistry
    B.S. Lanzhou University,Lanzhou, P. R. China, 1998, Chemistry

    Professional Experiences
  • 2011-Present, BIOPIC of Peking University,Research Associate Professor

    2009-2011, Harvard University, Postdoctoral Scholar in Chemistry

    2006-2009, West Virginia University, Postdoctoral Scholar in Chemistry

    1998-2001, Jinhua Chemical Co. Ltd, Liaoning, P. R. China. Assistant Engineer

    Research Interests
  • Massive parallel DNA sequencing is highly demanded in modern biological investigation. We are focusing on the development of multiplex sequencing-by-synthesis method that combines terminal-phosphate labeled fluorogenic nucleotides (TPLFNs) and resealable microreactors. In the presence of phosphatase, the incorporation of a non-fluorescent TPLFN into a primer strand of DNA by DNA polymerase results in the generation of a fluorescent dye molecule. This allows DNA sequencing in a manner akin to pyrosequencing but with a fluorescence signal. Also, BIOPIC has the world best research facilities and talent people to conduct single molecular study. The applications of synthetic chemistry, structural design, and numerous other chemical principles will provide many novel molecular probes which have great potential to be used as indicators for single molecular enzymology study.
    Awards and Honors
    Selected Publication
    • 9. Sims, P. A.; Greenleaf, W. J.; Duan, H.; Xie, X. “Fluorogenic Pyrosequencing in PDMS Microreactors” Nature Method. 2011 (accepted)
      8. Duan, H.; Sengupta, S.; Petersen, J. L.; Akhmedov, N.; Shi, X. "Triazole-Au(I) Complexes: A New Class of Catalysts with Improved Thermal Stability and Reactivity for Intermolecular Alkyne Hydroamination" Journal of the American Chemical Society, 2009, 131, 15387–15393.
      7. Duan, H.; Yan, W.; Sengupta, S.; Shi, X. "Highly Efficient Synthesis of Vinyl Substituted Triazoles by Au(I) Catalyzed Alkyne Activation " Bioorganic and Medicinal Chemistry Letters 2009, 3899-3902.
      6. Duan, H.; Sun, X. H.; Liao, W. Y.; Petersen, J. L.; Shi, X. "Proline as Lewis Base Catalyst: Diastereoselective Synthesis of Isoxazoline-N-Oxide through [3+2] Cycloaddition". Organic Letters 2008, 10, 4113-4116..
      5. Duan, H.; Xie, J.; Qiao, X.; Wang, L.; Zhou, Q. "Enantioselective Rhodium-catalyzed Addition of Arylboronic Acids to a-Ketoesters". Angewandte Chemie International Edition 2008, 47, 4351-4353.
      4. Yang, Y.; Zhu, S.; Duan, H.; Zhou, C.; Wang, L.; Zhou, Q. "Asymmetric Reductive Coupling of Dienes and Aldehydes Catalyzed by Nickel Complexes of Spiro Phosphoramidites: Highly Enantioselective Synthesis of Chiral Bishomoallylic Alcohols" Journal of the American Chemical Society 2007, 129, 2848-2849.
      3. Duan, H.; Jia, Y.; Wang, L.; Zhou, Q. "Enantioselecitive Rh-Catalyzed Arylation of N-Tosylarylimines with Arylboronic Acids". Organic Letters 2006, 8, 2567-2569.
      2. Duan, H.; Xie, J.; Shi, W.; Zhang, Q.; Zhou, Q. "Enantioselective Rhodium-Catalyzed Addition of Arylboronic Acids to Aldehydes Using Chiral Spiro Monophosphite Ligands". Organic Letters 2006, 8, 1479-1481..
      1. Xie, J.; Wang, L.; Fu, Y.; Zhu, S.; Fan, B.; Duan, H.; Zhou, Q. "Synthesis of Spiro Diphosphines and Their Application in Asymmetric Hydrogenation of Ketones". Journal of the American Chemical Society 2003, 125, 4404-4405