HOME» PEOPLE» Wensheng Wei

Wensheng Wei
Professor (PI)

    Professional Experiences

  • 2015-Present, Peking University-Tsinghua University Center for Life Sciences, Principle Investigator

    2014-Present, Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing, China, Principle Investigator

    2007- Present, College of Life Sciences and the State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing, China, Principle Investigator

    2005-2007, Prof. Stanley Cohen Lab, Department of Genetics, Stanford University School of Medicine, Stanford University, USA, Research Associate

    1999-2004, Prof. Stanley Cohen Lab, Department of Genetics, Stanford University School of Medicine Stanford University, USA, Postdoc

    1991-1993, Department of Biology, Peking University, China, Research Assistant
    Research Interests

  • Wei group is interested in studying the molecular mechanisms of human diseases, especially the host response to microbial pathogenicity. The combination of forward and reverse genetic means is employed, often in a high-throughput fashion, for the identification of host genes of interest. Efforts have also been put into the development and application of eukaryotic gene editing techniques, such as TALENs and CRISPR/Cas9 systems. The current focuses of diseases include Clostridium difficile and HCV infections.
    Awards and Honors

  • 2014,Bayer Investigator Award

    2014, Roche Chinese Young Investigator Award

    2012,Peking University Dongbao Fellowship

    2010,Most popular teacher of College of Life Sciences award

    Selected Publication

    • 15. Fan H, Qiao L, Kang KD, Fan J, Wei W and Luo G. Attachment and Postattachment Receptors Important for Hepatitis C Virus Infection and Cell-to-Cell Transmission. Journal of virology. 2017;91.
      14. Zhang Y, Liu L, Guo S, Song J, Zhu C, Yue Z, Wei W and Yi C. Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition. Nat Commun. 2017;8:901.
      13. Zhu S, Zhou Y and Wei W. Genome-Wide CRISPR/Cas9 Screening for High-Throughput Functional Genomics in Human Cells. Methods in molecular biology (Clifton, NJ). 2017;1656:175-181.
      12. He R, Peng J, Yuan P, Yang J, Wu X, Wang Y and Wei W. Glucosyltransferase Activity of Clostridium difficile Toxin B Triggers Autophagy-mediated Cell Growth Arrest. Sci Rep. 2017;7:10532.
      11. Hu H, Zhang H, Wang S, Ding M, An H, Hou Y, Yang X, Wei W, Sun Y and Tang C. Live visualization of genomic loci with BiFC-TALE. Sci Rep. 2017;7:40192.
      10. Zhou Y, Wang P, Tian F, Gao G, Huang L, Wei W and Xie XS. Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging. Cell Res. 2017;27:298-301.

    • 9. He R, Peng J, Yuan P, Xu F, Wei W*. (2015) Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy (in press).

    • 8. Yuan P, Zhang H, Cai C, Zhu S, Zhou Y, Yang X, He R, Li C, Guo S, Li S, Huang T, Feng H and Wei W*. (2014) Identification of the Cellular Receptor for Clostridium difficile Toxin B. Cell Res. doi: 10.1038/cr.2014.169.

    • 7. Zhou Y, Zhu S, Cai C, Yuan P, Li C, Huang Y and Wei W*. (2014) High-throughput Screening of a CRISPR/Cas Library for Functional Genomics in Human Cells. Nature 509(7501): 487-91.

    • 6. Qian LL, Cai CZ, Yuan PF, Jeong SY, Yang XZ, Almeida VD, Ernst J, Costa M, Cohen SN, Wei WS*. Bidirectional effect of Wnt signaling antagonist DKK1 on the modulation of anthrax toxin uptake. Sci China Life Sci, 2014, 57: 469–481.

    • 5. Yang J, Zhang Y, Yuan P, Cai C, Ren Q, Guo S, Zhu C, Qi H and Wei W*. (2014) A Complete Decoding of DNA Recognition by TAL Effector RVDs. Cell Res. 24:628-631.

    • 4. Yang J, Yuan P, Wen D , Sheng Y, Zhu S, Yu Y, Gao X and Wei W*. (2013) ULtiMATE system for rapid assembly of customized TAL effectors. PLoS One 2013; 8:e75649.

    • 3. Wei W*, Lu Q., Chaudry J, Leppla S, Cohen SN. (2006) The LDL receptor-related protein LRP6 mediates internalization and lethality of anthrax toxin. Cell 124, 1141-1154.

    • 2. Wei W*, PlovanichJones A, Deng W, Jin Q, Collmer A, Huang H, and He SY. (2000) The gene coding for the Hrp pilus structural protein is required for type III secretion of Hrp and Avr proteins in Pseudomonas syringae pv. tomato. Proc. Natl. Acad. Sci. USA 97, 2247-52.

    • 1. Roine E, Wei W*, Yuan J, Nurmiaho-Lassila EL, Kalkkinen N, Romantschuk M, He SY. (1997) Hrp pilus: an hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000. Proc. Natl. Acad. Sci. USA 94, 3459-64. (*Co-first author)