Yujie Sun
Assistant Professor (PI)

  • E-mail:single_molecule@pku.edu.cn
  • Tel:+86-10-62747460
    Office:Rm. 203 BIOPIC Building
    Mailing Address:Biodynamics Optical Imaging Center, Peking University, Beijing, China, 100871
  • PhD,University of Pittsburgh,2005,Chemistry
    MS,University of Sci. & Tech. China,1999,Chemistry
    BS,University of Sci. & Tech. China,1996,Chemistry

    Professional Experiences

  • 2010-now Assistant Professor, Biodynamics Optical Imaging Center, College of Life Sciences & Adjunct Assistant Professor, College of Chemistry and Molecular Engineering, Peking University

    2005-2010,Postdoctoral Fellow & Senior Investigator, Pennsylvania Muscle Institute & Nano/Bio Interface Center, University of Pennsylvania
    Research Interests

  • Single molecule techniques have become the essential means for life sciences. One of their ultimate goals is to observe, in a live cell at real time, the dynamics of single molecules, including bio-macromolecules, thereby at the molecular level, to understand cellular processes under the physiological condition. The main direction of the Lab of Single Molecule and Live Cell Dynamics is to invent, develop, and apply new and present single molecule techniques, including super-resolution single molecule fluorescence microscopy and single molecule manipulation, and to study the gene regulation and expression of live cells, such as cancer cells and stem cells, at the molecular cell and advance the research of cancer therapy and stem cell. Collaborating with other PIs at BIOPIC, we are going to push forward the following research directions using super-resolution imaging and live-cell imaging techniques:(1) Gene regulation and expression of mammals; (2) The role of stochastic gene expression in stem cell differentiation; (3) Using flow cytometry, microfluidics, and high-throughput sequencing, we will advance our understanding of gene regulation from the level of single cell to the level of cell colony, tissue, and organs, and understand at the higher order of life, how gene regulation and expression are coordinated among cells.
    Awards and Honors

  • 2017, Bayer Investigator Award

    2014, Tonghuadongbao Teacher's Award, College of Biology, Peking University

    2012, Youth Qianren Program, Ministry of Science and Technology
    2010, Best Poster Award, PMI Annual Meeting, University of Pennsylvania
    2007,Best Poster Award, Physiology Department Annual Meeting, University of Pennsylvania
    2006,Best Poster Award, Physiology Department Annual Meeting, University of Pennsylvania
    2006 ,Postdoctoral Travel Award, Biophysical Society, Molecular Motor Discussions Meeting
    2000 ,Safford Award for Excellence as a Graduate Student Teacher, University of Pittsburgh

    Selected Publication

    29. Guan R, Zhang L, Su QP, Mickolajczyk KJ, Chen GY, Hancock WO, Sun Y, Zhao Y and Chen Z. Crystal structure of Zen4 in the apo state reveals a missing conformation of kinesin. Nat Commun. 2017;8:14951.

    28. Wang S, Ding M, Chen X, Chang L and Sun Y. Development of bimolecular fluorescence complementation using rsEGFP2 for detection and super-resolution imaging of protein-protein interactions in live cells. Biomed Opt Express. 2017;8:3119-3131.
    27. Shao S, Chang L, Hou Y and Sun Y. Illuminating the structure and dynamics of chromatin by fluorescence labeling. Frontiers in Biology. 2017;12:241-257.
    26. Wang S, Ding M, Xue B, Hou Y and Sun Y. Live Cell Visualization of Multiple Protein–Protein Interactions with BiFC Rainbow. ACS Chemical Biology. 2017.
    25. Hu H, Zhang H, Wang S, Ding M, An H, Hou Y, Yang X, Wei W, Sun Y and Tang C. Live visualization of genomic loci with BiFC-TALE. Sci Rep. 2017;7:40192.
    24. Fang X, Chen X, Li R, Liu Z, Chen H, Sun Z, Ju B, Liu Y, Zhang S, Ding D, Sun Y and Wu C. Multicolor Photo‐Crosslinkable AIEgens toward Compact Nanodots for Subcellular Imaging and STED Nanoscopy. Small. 2017.
    23. Chen X, Liu Z, Li R, Shan C, Zeng Z, Xue B, Yuan W, Mo C, Xi P, Wu C and Sun Y. Multicolor Super-resolution Fluorescence Microscopy with Blue and Carmine Small Photoblinking Polymer Dots. ACS Nano. 2017;11:8084-8091.
    22. Shao S, Chang L, Sun Y, Hou Y, Fan X and Sun Y. Multiplexed sgRNA Expression Allows Versatile Single Non-repetitive DNA Labeling and Endogenous Gene Regulation. bioRxiv. 2017.
    21. Chen X, Li R, Liu Z, Sun K, Sun Z, Chen D, Xu G, Xi P, Wu C and Sun Y. Small Photoblinking Semiconductor Polymer Dots for Fluorescence Nanoscopy. Adv Mater. 2017;29.
    20. Han X, Li P, Yang Z, Huang X, Wei G, Sun Y, Kang X, Hu X, Deng Q, Chen L, He A, Huo Y, Li D, Betzig E and Luo J. Zyxin regulates endothelial von Willebrand factor secretion by reorganizing actin filaments around exocytic granules. Nat Commun. 2017;8:14639.

    19. Xuanze Chen*, Rongqin Li*, Zhihe Liu*, Kai Sun, Zezhou Sun, Danni Chen, Gaixia Xu, Peng Xi, Changfeng Wu,* & Yujie Sun*, Photoblinking small semiconductor polymer dots for fluorescence nanoscopy, Adv Mater, 2016. 

    18. Sheng Wang*, Xuanze Chen*, Lei Chang*, Ruiying Xue, Haifeng Duan, and Yujie Sun,GMars-Q Enables Long-Term Live-Cell Parallelized Reversible Saturable Optical Fluorescence Transitions Nanoscopy,ACS Nano, 2016, 10:9136–9144

    17. Xuanze Chen*, Zhiping Zeng*, Rongqin Li, Boxin Xue, Peng Xi*, and Yujie Sun*, Superior performance with sCMOS over EMCCD in Super-Resolution Optical Fluctuation Imaging (SOFI), J Biomed Optics, 2016, 6:066007

    16. Xuanze Chen*, Weijian Zong,Rongqin Li, Zhiping Zeng, Jia Zhao, Peng Xi, Liangyi Chen and Yujie Sun, Two-photon light-sheet nanoscopy by fluorescence fluctuation correlation analysis, Nanoscale, 2016, 8:9982–9987.

    15.Xuanze Chen*, Mian Wei*, M. Mocarlo Zheng*, Jiaxi Zhao, Huiwen Hao, Lei Chang, Peng Xi, and Yujie Sun, Study of RNA Polymerase II Clustering inside Live-Cell Nuclei Using Bayesian Nanoscopy, ACS Nano, 2016, 10:2447–54.

    14.Wanqing Du*, Qian Peter Su*, Yang Chen*, Yueyao Zhu, Dong Jiang, Yueguang Rong, Senyan Zhang, Yixiao Zhang, He Ren, Chuanmao Zhang, Xinquan Wang, Ning Gao, Yanfeng Wang, Lingfei Sun, Yujie Sun* and Li Yu*, Kinesin 1 Drives Autolysosome Tubulation, Dev Cell, 2016, 37:326–336

    13.Qian Peter Su*, Wanqing Du*, Qinghua Ji, Boxin Xue, Dong Jiang, Yueyao Zhu, Jizhong Lou, Li Yu*, and Yujie Sun*, Vesicle Size Regulates Nanotube Formation in the Cell, Sci Rep, 2016, 6:24002

    12. Shipeng Shao, Weiwei Zhang, Huan Hu, Boxin Xue, Jinshan Qin, Chaoying Sun, Yuao Sun, Wensheng Wei, Yujie Sun, Long-term Dual-color Tracking of Genomic Loci by Modified sgRNAs of the CRISPR/Cas9 System, Nucleic Acids Res, 2016, 44:e86

    11.Wanqing Du, Qian Peter Su, Yang Chen, Yueyao Zhu, Dong Jiang, Yueguang Rong, Senyan Zhang, Yixiao Zhang, He Ren, Chuanmao Zhang, Xinquan Wang, Ning Gao, Yanfeng Wang, Lingfei Sun, Yujie Sun* and Li Yu*, Dev Cell, 2016, 37, 326–336 (*co-corresponding)

    10.Shipeng Shao, Weiwei Zhang, Huan Hu, Boxin Xue, Jinshan Qin, Chaoying Sun, Yuao Sun, Wensheng Wei, Yujie Sun, Long-term Dual-color Tracking of Genomic Loci by Modified sgRNAs of the CRISPR/Cas9 System, Nucleic Acids Res, 2016, Nucleic Acids Res., 2016, 44, e86.

    9.Sun, Y. (2014). Super-resolution imaging with stochastic optical reconstruction microscopy (STORM) and photoactivated localization microscopy (PALM) in Optical Nanoscopy and Novel Microscopy Techniques, Xi, P., eds. (CRC Press) 

    8. Chong Wang · Wanqing Du · Qian Peter Su · Mingli Zhu · Peiyuan Feng · Ying Li · Yichen Zhou · Na Mi · Yueyao Zhu · Dong Jiang · Senyan Zhang · Zerui Zhang · Yujie Sun* · Li Yu*,Dynamic tubulation of mitochondria drives mitochondrial network formation,Cell Res. 2015 In press (*co-corresponding) 

    7. Weijian Zong, Jia Zhao, Xuanyang Chen, Yuan Lin, Huixia Ren, Yunfeng Zhang, Ming Fan, Zhuan Zhou, Heping Cheng, Yujie Sun* and Liangyi Chen*, Large-field high-resolution two-photon digital scanned light-sheet microscopy, Cell Res.,2014,5:1-4 (*co-corresponding) 

    6. Zhen Liu, Dong Xing, Qian Su, Xinyu Kong, Jiamei Zhang, Boxin Xue, Hao Sun, Sheng Wang, Yile Tao, Yujie Sun, Super-resolution Imaging and Tracking of Protein-Protein Interaction in Subdiffraction Cellular Space, Nature Comm. 2014,5:4443-4449 

    5. Sangjin Kim&, Erik Brostr?mer&, Dong Xing&, Jianshi Jin&, Shasha Chong, HaoGe, Siyuan Wang, Chan Gu, Lijiang Yang, Yi Qin Gao, Xiao-dong Su*, Yujie Sun*, X. SunneyXie*, Probing Allostery Through DNA. Science 2013, 339:816-819 (*co-corresponding) 

    4.  Yujie Sun, Osamu Sato, Felix Ruhnow, Mark E. Arsenault, Mitsuo Ikebe, and Yale E. Goldman. Single Molecule Stepping and Structural Dynamics of Myosin X. Nat Struct Mol Biol 2010,17, 485-491 . 

    3.  Yujie Sun, Jennine D. McKenna, John M. Murray, E. Michael Ostap and Yale E. Goldman. Parallax: High Accuracy Three-Dimensional Single Molecule Tracking Using Split Images. Nano Lett 2009, 9, 2676-2682. 

    2. Yujie Sun, Harry W. Schroeder III, John F. Beausang, Kazuaki Homma, Mitsuo Ikebe, and Yale E. Goldman. Myosin VI walks “wiggly” on actin with large and variable tilting. Mol Cell 2007, 28, 954-964. 

    1. Yujie Sun, Boris Akhremitchev, Gilbert C. Walker. Using the Adhesive Interaction between AFM tips and Polymer Surfaces to Measure the Elastic Modulus of Compliant Samples. Langmuir 2004, 20, 5837-5845.