Biomedical Pioneering Innovation Center
北大主站 中文 切换旧版
北大主站 中文 切换旧版
Home» Research Team»
Back
Contact

E-mail:sunneyxie@pku.edu.cn
Tel:+86-010-62744061
Add:Rm.107, Biomedical Pioneering Innovation Center, Integrated Science Research Center No.2,Peking University
Mailing Address:BIOPIC, Integrated Science Research Center No.2, Peking University, Yiheyuan Rd., No. 5, Haidian District, Beijing, 100871

Lab Web：http://sunneyxielab.org/

Xiaoliang Sunney Xie
Lee Shau Kee Professor;Director,ICG
Resume

2020-Present, Director, Changping Laboratory

2019.11-Present, Dean of Sciences, Peking University

2018.7-Present, Lee Shau-kee Professor, Peking University

2016.8-Present, Director, Beijing Advanced Innovation Center for Genomics

2011-Present, PI, PKU-Tsinghua Center for Life Sciences

2010-2022.03, Founder and Director, Biodynamic Optical Imaging Center (now named Biomedical Pioneering Innovation Center), Peking University

2010-Present, Adjunct Professor of Chemistry, School of Chemistry and Molecular Engineering, Peking University

2009-Present, Cheung Kong Visiting Professor of Life Sciences, School of Life Sciences, Peking University

2009-2018, Mallinckrodt Professor of Chemistry and Chemical Biology, Department of Chemistry and Chemical Biology, Harvard University

1999-2008, Professor of Chemistry, Harvard University, Department of Chemistry and Chemical Biology

1992-1998, Senior Research Scientist to Chief Scientist, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory

1990-1992, Postdoctoral Fellow, University of Chicago

1990, Ph.D, University of California at San Diego Chemistry, Chemistry, 1990

1984, B.S. Peking University, Beijing, P. R. China, Chemistry

Research Interests

Prof. Xie’s current research interests include: 

(1) Development of single-cell sequencing technology: single-cell genome sequencing, transcriptome sequencing, methylation sequencing, chromatin three-dimensional result analysis, chromatin openness detection and single-cell multiomics.  (2) Application of single cell sequencing technology in gene expression regulation: mechanism of gene expression regulated by transcription factors, the dynamic changes of epigenetic modifications during early embryonic development, how epigenetic coordination affects the construction of gene regulation network, etc.  (3) Application of single cell sequencing technology in medical Transformation: early cancer screening and diagnosis, preimplantation genetic screening and diagnosis in in vitro fertilization, high-throughput antibody drug screening, etc.

Awards and Honors

2025 Beijing Natural Science Award, First Prize

2024 Tengchong Science Award

2021 Zhongguancun Award for Outstanding Contributions

2017 Member of the Chinese Academy of Sciences

2017 Qiushi Prize

2016 Ray Wu Award, Chinese Biological Investigators Society

2015 Shizhang Bei Prize in Biophysics, Chinese Biophysical Society

2015 Albany Prize in Medicine Recipient

2015 ACS Peter Debye Award in Physical Chemistry

2014 Fellow of Optical Society of America

2014 Humboldt Fellowship, Germany

2013 NIH Director's Pioneer Award Recipient

2013 Ellis R. Lippincott Award, Optical Society of America and Society for Applied Spectroscopy

2012 Harrison Howe Award, Rochester Section of American Chemical Society

2012 Fellow of the American Academy of Microbiology

2012 Biophysical Society Founders Award

2010 Honorary Fellow of the Chinese Chemical Society

Selected Publication

Single Cell Genomics

  1. Wu, H.; Zhang, J.; Tan, L.; Xie, X. Single-cell Micro-C profiles 3D genome structures at high resolution and characterizes multi-enhancer hubs. Nat Genet. 2025, 57, 1777–1786. 10.1038/s41588-025-02247-6 

  2. He, R.; Dong, W.; Wang, Z.; Xie, C.; Gao, L.; Ma, W.; Shen, K.; Li, D.; Pang, Y.; Jian, F.; Zhang, J.; Yuan, Y.; Wang, X.; Zhang, Z.; Zhen, Y.; Liu, S.; Luo, C.; Chai, X.; Ren, J.; Zhu, Z.; Xie, X. Genome-wide single-cell and single-molecule footprinting of transcription factors with deaminase. PNAS. 2024, 121(52), e2423270121. 10.1073/pnas.242327012 

  3. Chapman, A.; Lee, D.; Cai, W.; Ma, W.; Li, X.; Sun, W.; Xie, X. Correlated gene modules uncovered by high-precision single-cell transcriptomics. PNAS. 2022, 119(51), e2206938119. 10.1073/pnas.2206938119 

  4. Tan, L.; Ma, W.; Wu, H.; Sun, W.; Zheng, Y.; Xing, D.; Chen, R.; Li, X.; Daley, N.; Deisseroth, K.; Xie, X. Changes in genome architecture and transcriptional dynamics progress independently of sensory experience during post-natal brain development. Cell. 2021, 184(3), 741-758. 10.1016/j.cell.2020.12.032 

  5. Tan, L.; Xing, D.; Chang, C.; Li, H.; Xie, X. Three-dimensional genome structures of single diploid human cells. Science. 2018, 361, 924-928. 10.1126/science.aat5641 

  6. Chen, C.; Xing, D.; Tan, L.; Li, H.; Zhou, G.; Huang, L.; Xie, X. Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI). Science. 2017, 356, 189-194. 10.1126/science.aak9787 

  7. Yan, L.; Huang, L.; Xu, L.; Huang, J.; Ma, F.; Zhu, X.; Tang, Y.; Liu, M.; Lian, Y.; Liu, P.; Li, R.; Lu, S.; Tang, F.; Qiao, J.; Xie, X. Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses. PNAS. 2015, 112(52), 15964-15969. 10.1073/pnas.1523297113 

  8. Hou, Y.; Fan, W.; Yan, L.; Li, R.; Lian, Y.; Huang, J.; Li, J.; Xu, L.; Tang, F.; Xie, X.; Qiao, J. Genome Analyses of Single Human Oocytes. Cell. 2013, 155(7), 1492-1506. 10.1016/j.cell.2013.11.040 

  9. Ni, X.; Zhuo, M.; Su, Z.; Duan, J.; Gao, Y.; Wang, Z.; Zong, C.; Bai, H.; Chapman, A.; Zhao, J.; Xu, L.; An, T.; Ma, Q.; Wang, Y.; Wu, M.; Sun, Y.; Wang, S.; Li, Z.; Yang, X.; Yong, J.; Su, X.; Lu, Y.; Bai, F.; Xie, X.; Wang, J. Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients. PNAS. 2013, 110(52), 21083-21088. 10.1073/pnas.1320659110 

  10. Zong, C.; Lu, S.; Chapman, A.; Xie, X. Genome-Wide Detection of Single-Nucleotide and Copy-Number Variations of a Single Human Cell. Science. 2012, 338(6114), 1622-1626. 10.1126/science.1229164 

  11. Lu, S.; Zong, C.; Fan, W.; Yang, M.; Li, J.; Chapman, A.; Zhu, P.; Hu, X.; Xu, L.; Yan, L.; Bai, F.; Qiao, J.; Tang, F.; Li, R.; Xie, X. Probing Meiotic Recombination and Aneuploidy of Single Sperm Cells by Whole-Genome Sequencing. Science. 2012, 338(6114), 1627-1630. 10.1126/science.1229112 

  12. Shiroguchi, K.; Jia, T.; Sims, P.; Xie, X. Digital RNA sequencing minimizes sequence-dependent bias and amplification noise with optimized single-molecule barcodes. PNAS. 2012, 109(4), 1347-1352. 10.1073/pnas.1118018109 

  13. Sims, P.; Greenleaf, W.; Duan, H.; Xie, X. Fluorogenic DNA sequencing in PDMS microreactors. Nat Meth. 2011, 8(7), 575-580. 10.1038/nmeth.1629 

Coherent Raman Microscopy

  1. Ji, M.; Orringer, D.; Freudiger, C.; Ramkissoon, S.; Liu, X.; Lau, D.; Golby, A.; Norton, I.; Hayashi, M.; Agar, N.; Young, G.; Spino, C.; Santagata, S.; Camelo-Piragua, S.; Ligon, K.; Sagher, O.; Xie, X. Rapid, Label-Free Detection of Brain Tumors with Stimulated Raman Scattering Microscopy. Science Translational Medicine. 2013, 5(201), 201ra119-201ra119. 10.1126/scitranslmed.3005954 

  2. Wang, M.; Min, W.; Freudiger, C.; Ruvkun, G.; Xie, X. RNAi screening for fat regulatory genes with SRS microscopy. Nat Meth. 2011, 8(2), 135-138. 10.1038/nmeth.1556 

  3. Saar, B.; Freudiger, C.; Reichman, J.; Stanley, C.; Holtom, G.; Xie, X. Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering. Science. 2010, 330(6009), 1368-1370. 10.1126/science.1197236 

  4. Freudiger, C.; Min, W.; Saar, B.; Lu, S.; Holtom, G.; He, C.; Tsai, J.; Kang, J.; Xie, X. Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy. Science. 2008, 322(5909), 1857-1861. 10.1126/science.1165758 

  5. Evans, C.; Potma, E.; Puoris'haag, M.; Cote, D.; Lin, C.; Xie, X. Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy. PNAS. 2005, 102(46), 16807-16812. 10.1073/pnas.0508282102 

  6. Zumbusch, A.; Holtom, C.; Xie, X. Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering. Physical Review Letters. 1999, 82, 4142-4145.  

Gene Expression and Regulation - Live Cell Single Molecule Studies

  1. Taniguchi, Y.; Choi, P.; Li, G.; Chen, H.; Babu, M.; Hearn, J.; Emili, A.; Xie, X. Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells. Science. 2010, 329(5991), 533-538. 10.1126/science.1188308 

  2. Choi, P.; Cai, L.; Frieda, K.; Xie, X. A Stochastic Single-Molecule Event Triggers Phenotype Switching of a Bacterial Cell. Science. 2008, 322(5900), 442-446. 10.1126/science.1161427 

  3. Elf, J.; Li, G.; Xie, X. Probing Transcription Factor Dynamics at the Single-Molecule Level in a Living Cell. Science. 2007, 316(5828), 1191-1194. 10.1126/science.1141967 

  4. Yu, J.; Xiao, J.; Lao, K.; Xie, X. Probing Gene Expression in Live Cells, One Protein Molecule at a Time. Science. 2006, 311(5767), 1600-1603. 10.1126/science.1119623 

  5. Cai, L.; Friedman, N.; Xie, X. Stochastic protein expression in individual cells at the single molecule level. Nature. 2006, 440(7082), 358-362. 10.1038/nature04599 

Single Molecule Enzymology

  1. Chong, S.; Chen, C.; Ge, H.; Xie, X. Mechanism of Transcriptional Bursting in Bacteria. Cell. 2014, 158(2), 314-326. 10.1016/j.cell.2014.05.038 

  2. Kim, S.; Brostromer, E.; Xing, D.; Jin, J.; Chong, S.; Ge, H.; Wang, S.; Gu, C.; Yang, L.; Gao, Y.; Su, X.; Sun, Y.; Xie, X. Probing Allostery Through DNA. Science. 2013, 339(6121), 816-819. 10.1126/science.1229223 

  3. English, B.; Min, W.; van Oijen, A.; Lee, K.; Luo, G.; Sun, H.; Cherayil, B.; Kou, S.; Xie, X. Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited. Nat Chem Biol. 2006, 2(2), 87-94. 10.1038/nchembio759 

  4. Yang, H.; Luo, G.; Karnchanaphanurach, P.; Louie, T.; Rech, I.; Cova, S.; Xun, L.; Xie, X. Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer. Science. 2003, 302(5643), 262-266. 10.1126/science.1086911 

  5. Lu, H.; Xun, L.; Xie, X. Single Molecule Enzymatic Dynamics. Science. 1998, 282, 1877.  

Single Molecule Spectroscopy and Imaging

  1. Chong, S.; Min, W.; Xie, X. Ground-State Depletion Microscopy: Detection Sensitivity of Single-Molecule Optical Absorption at Room Temperature. The Journal of Physical Chemistry Letters. 2010, 1(23), 3316-3322. 10.1021/jz1014289 

  2. Min, W.; Lu, S.; Chong, S.; Roy, R.; Holtom, G.; Xie, X. Imaging chromophores with undetectable fluorescence by stimulated emission microscopy. Nature. 2009, 461(7267), 1105-1109. 10.1038/nature08438 

  3. Sánchez, E.; Novotny, L.; Xie, X. Near-field Fluorescence Microscopy Based on Two-Photon Excitation With Metal Tips. Phys. Rev. Lett. 1999, 82, 4014.  

  4. Lu, H.; Xie, X. Single-Molecule Spectral Fluctuations at Room Temperature. Nature. 1997, 385, 143.  

  5. Sánchez, E.; Novotny, L.; Holtom, G.; Xie, X. Room-Temperature Fluorescence Imaging and Spectroscopy of Single Molecules by Two-Photon Excitation. J. Phys. Chem. A. 1997, 101, 7019.  

  6. Xie, X.; Dunn, R. Probing Single Molecule Dynamics. Science. 1994, 265, 361.  

COVID-19

  1. Cao, Y.; Jian, F.; Wang, J.; Yu, Y.; Song, W.; Yisimayi, A.; Wang, J.; An, R.; Chen, X.; Zhang, N.; Wang, Y.; Wang, P.; Zhao, L.; Sun, H.; Yu, L.; Yang, S.; Xiao, T.; Gu, Q.; Shao, F.; Hao, X.; Xu, Y.; Jin, R.; Shen, Z.; Wang, Y.; Xie, X. Imprinted SARS-CoV-2 humoral immunity induces convergent Omicron RBD evolution. Nature. 2023, 614, 521-529. 10.1038/s41586-022-05644-7 

  2. Cao, Y.; Jian, F.; Zhang, Z.; Yisimayi, A.; Hao, X.; Bao, L.; Yuan, F.; Yu, Y.; Du, S.; Wang, J.; Xiao, T.; Song, W.; Zhang, Y.; Liu, P.; An, R.; Wang, P.; Wang, Y.; Yang, S.; Niu, X.; Zhang, Y.; Gu, Q.; Shao, F.; Hu, Y.; Yin, W.; Zheng, A.; Wang, Y.; Qin, C.; Jin, R.; Xiao, J.; Xie, X. Rational identification of potent and broad sarbecovirus-neutralizing antibody cocktails from SARS convalescents. Cell Reports. 2022, 41(12), 111845. 10.1016/j.celrep.2022.111845 

  3. Cao, Y.; Song, W.; Wang, L.; Liu, P.; Yue, C.; Jian, F.; Yu, Y.; Wang, Y.; Yisimayi, A.; Wang, P.; Wang, Y.; Zhu, Q.; Deng, J.; Fu, W.; Yu, L.; Zhang, N.; Wang, J.; Xiao, T.; An, R.; Wang, J.; Liu, L.; Yang, S.; Niu, X.; Gu, Q.; Shao, F.; Hao, X.; Meng, B.; Gupta, R.; Jin, R.; Wang, Y.; Xie, X.; Wang, X. Characterization of the enhanced infectivity and antibody evasion of Omicron BA.2.75. Cell Host & Microbe. 2022, 30(11), 1527-1539. 10.1016/j.chom.2022.09.018 

  4. Cao, Y.; Yisimayi, A.; Jian, F.; Song, W.; Xiao, T.; Wang, L.; Du, S.; Wang, J.; Wang, J.; Li, Q.; Chen, X.; Yu, Y.; Wang, P.; Zhang, Z.; Liu, P.; An, R.; Hao, X.; Wang, Y.; Wang, J.; Feng, R.; Sun, H.; Zhao, L.; Zhang, W.; Zhao, D.; Zheng, J.; Yu, L.; Li, C.; Zhang, N.; Wang, R.; Niu, X.; Yang, S.; Song, X.; Chai, Y.; He, Y.; Shi, Y.; Zheng, L.; Li, Z.; Gu, Q.; Shao, F.; Huang, W.; Jin, R.; Shen, Z.; Wang, Y.; Wang, X.; Xiao, J.; Xie, X. BA.2.12.1, BA.4 and BA.5 escape antibodies elicited by Omicron infection. Nature. 2022, 608, 593-602. 10.1038/s41586-022-04980-y 

  5. Cao, Y.; Wang, J.; Jian, F.; Xiao, T.; Song, W.; Yasimayi, A.; Huang, W.; Li, Q.; Wang, P.; An, R.; Wang, J.; Wang, Y.; Niu, X.; Yang, S.; Liang, H.; Sun, H.; Li, T.; Yu, Y.; Cui, Q.; Liu, S.; Yang, X.; Du, S.; Zhang, Z.; Hao, X.; Shao, F.; Jin, R.; Wang, X.; Xiao, J.; Wang, Y.; Xie, X. Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodies. Nature. 2021, 602, 657-663. 10.1038/s41586-021-04385-3 

  6. Cao, Y.; Yisimayi, A.; Bai, Y.; Huang, W.; Li, X.; Zhang, Z.; Yuan, T.; An, R.; Wang, J.; Xiao, T.; Du, S.; Ma, W.; Song, L.; Li, Y.; Li, X.; Song, W.; Wu, J.; Liu, S.; Li, X.; Zhang, Y.; Su, B.; Guo, X.; Wei, Y.; Gao, C.; Zhang, N.; Zhang, Y.; Dou, Y.; Xu, X.; Shi, R.; Lu, B.; Jin, R.; Ma, Y.; Qin, C.; Wang, Y.; Feng, Y.; Xiao, J.; Xie, X. Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines. Cell Research. 2021, 31(7), 732-741. 10.1038/s41422-021-00514-9 

  7. Du, S.; Cao, Y.; Zhu, Q.; Yu, P.; Qi, F.; Wang, G.; Du, X.; Bao, L.; Deng, W.; Zhu, H.; Liu, J.; Nie, J.; Zheng, Y.; Liang, H.; Liu, R.; Gong, S.; Liu, J.; Xu, H.; Yisimayi, A.; Lv, Q.; Wang, B.; He, R.; Han, Y.; Zhao, W.; Bai, Y.; Qu, Y.; Gao, X.; Ji, C.; Wang, Q.; Gao, N.; Huang, W.; Wang, Y.; Xie, X.; Su, X.; Xiao, J.; Qin, C. Structurally resolved SARS-CoV-2 antibody shows high efficacy in severely infected hamsters and provides a potent cocktail pairing strategy. Cell. 2020, 183(4), 1013-1023.e13. 10.1016/j.cell.2020.09.035 

  8. Cao, Y.; Su, B.; Guo, X.; Sun, W.; Deng, Y.; Bao, L.; Zhu, Q.; Zhang, X.; Zheng, Y.; Geng, C.; Chai, X.; He, R.; Li, X.; Lv, Q.; Zhu, H.; Deng, W.; Xu, Y.; Wang, Y.; Qiao, L.; Tan, Y.; Song, L.; Wang, G.; Du, X.; Gao, N.; Liu, J.; Xiao, J.; Su, X.; Du, Z.; Feng, Y.; Qin, C.; Qin, C.; Jin, R.; Xie, X. Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients' B Cells. Cell. 2020, 182(1), 73-84.e16. 10.1016/j.cell.2020.05.025 

BIOPIC QR code

ICG QR code

Facility
under construction
Education
under construction
Alumni
under construction
Links
Peking University School of Life Sciences Peking University Health Science Center

Mailing Address:Biomedical Pioneering Innovation Center,Peking University, Beijing, China, 100871

E-mail:contact@biopic.pku.edu.cn

Copyright ©  Biomedical Pioneering Innovation Center, Peking University