Add:Rm. B108, Biomedical Pioneering Innovation Center, Integrated Science Research Center No.2,Peking University

Lab Web:

Yanyi Huang

2022-Present, BIOPIC, Peking University, Deputy Director

2020-Present, College of Chemistry and Molecular Engineering, Peking University, Professor

2016-Present, Beijing Advanced Innovation Center for Genomics, Principal Investigator

2015-Present, Peking University-Tsinghua University Center for Life Sciences, Principle InvestigatorDeputy Director

2013-2020, College of Engineering, Peking University, Professor

2010-Present, BIOPIC, Peking University, Principle Investigator

2006-Present, College of Engineering, Peking University, Principle Invesetigator

2005-2006, Stanford University, Postdoctoral Scholar in Bioengineering

2002-2005, California Institute of Technology, Postdoctoral Scholar in Applied Physics

Research Interests

Huang group is working on technology development for integrative biology researches. Our current research interests include genome sequencing technologies, microfluidics, and bioimaging. We are developing a few key advanced technologies to facilitate genome sequencing and its applications, especially the novel chemistry and instruments for DNA sequencing, single-cell genome and transcriptome analysis, as well as epigenomic studies of limited number of cells. We also apply large-scale integrated microfluidic devices to perform quantitative biological studies. We use microfluidic chips to culture cells and to apply the stimuli precisely. By observing the cells' behavior on-chip, we are able to track the stochastic and dynamic life process with single-cell resolution. We are developing new imaging technologies and image processing algorithms to study the dynamics of cells and organisms. We use coherent Raman scattering microscopes to study the chemical distribution in living animals and cells without labeling. Currently we are also working on drug delivery and cancer diagnosis using this label-free technology.

Awards and Honors

2015, NSFC Outstanding Young Investigator Award

2014, Fellow of Royal Society of Chemistry (FRSC)

2013, Luye Biomedical Award for Outstanding Young Scholar

2012, NSFC Excellent Young Investigator Award

2010, Fok Ying Tung Education Foundation Young Investigator Award

2009, Peking University Teaching Contest, 1st Rank

2009, Teaching Contest for Junior University Teachers in Beijing City, 3rd Rank

2009, Teaching Contest for Junior University Teachers in Beijing City, Student-Favorite Award

2008, Program for New Century Excellent Talents in University, Ministry of Education, China

2004, National Excellent Doctoral Thesis Award, Ministry of Education, China

2003, National Natural Science Award, 2nd Rank, State Council, China

2002, Lee Wang Se Al Mei Scholarship

2001, May-Fourth Medal, Peking University

2001, Excellent Paper Award, 1st Rank, Beijing Chemical Society

2001, Excellent Paper Award, 1st Rank, Beijing Science and Technology Society

2001, Best Poster Award, Xiangshan Conference

2000, May-Fourth Scholarship, National Student Union

1999, Academic Top 10, Graduate Student Union, Peking University

1997, May-Fourth Youth Science Award, 1st Rank, Peking University

1997, Outstanding Graduate Award, Beijing City

1995, Outstanding Student Award, Peking University

1995, Dong Scholarship, Peking University

1995, May-Fourth Youth Science Award, 1st Rank, Peking University

1995, Outstanding Student Award, Beijing City

1993, National Chemistry Competition, Chinese Chemical Society, 1st Rank

1993, Outstanding Freshman Scholarship, Peking University

Selected Publication
  1. Ruoyan Li,# Lin Di,# Jie Li,# Wenyi Fan,# Yachen Liu, Wenjia Guo, Weiling Liu, Lu Liu, Qiong Li, Liping Chen, Yamei Chen, Chuanwang Miao, Hongjin Liu, Yuqian Wang, Yuling Ma, Deshu Xu, Dongxin Lin*, Yanyi Huang*, Jianbin Wang*, Fan Bai*, Chen Wu*. A body map of somatic mutagenesis in morphologically normal human tissues. Nature 2021, 597 (7876), 398-403.
  2. Lin Di, Yusi Fu, Yue Sun, Jie Li, Lu Liu, Jiacheng Yao, Guanbo Wang, Yalei Wu, Kaiqin Lao, Raymond W Lee, Genhua Zheng, Jun Xu, Juntaek Oh, Dong Wang, X. Sunney Xie*, Yanyi Huang*, Jianbin Wang*. RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids. PNAS 2020, 117, 2886-2893.

  3. Xiannian Zhang, Tianqi Li, Feng Liu, Yaqi Chen, Jiacheng Yao, Zeyao Li, Yanyi Huang, Jianbin Wang. VVV1. Comparative analysis of droplet-based ultra-high-throughput single-cell RNA-seq systems. Molecular Cell. 2019 73(13) 130-142.e5

  4. Geng, S (Geng, Shuang); Huang, YY (Huang, Yanyi).  From Mouth Pipetting to Microfluidics: The Evolution of Technologies for Picking Healthy Single Cells. ADVANCED BIOSYSTEMS.  2018  2(9)UNSP 1800099.
  5. Zhang, Y (Zhang, Yi); Gao, JX (Gao, Jiaxin); Huang, YY (Huang, Yanyi); Wang, JB (Wang, Jianbin). Recent Developments in Single-Cell RNA-Seq of Microorganisms.  BIOPHYSICAL JOURNAL,  2018 115(2)173-180.
  6. Jiang, Dongqing; Zhang, Xiannian; Pang, Yuhong; Zhang, Jianyun; Wang, Jianbin; Huang, Yanyi. Terminal transfer amplification and sequencing for high-efficiency and low-bias copy number profiling of fragmented DNA samples. Protein & cell.  2018.
  7. Chen, H (Chen, He); Yao, JC (Yao, Jiacheng); Fu, YS (Fu, Yusi); Pang, YH (Pang, Yuhong); Wang, JB (Wang, Jianbin); Huang, YY (Huang, Yanyi). Tagmentation on Microbeads: Restore Long-Range DNA Sequence Information Using Next Generation Sequencing with Library Prepared by Surface-Immobilized Transposomes. ACS APPLIED MATERIALS & INTERFACES.  2018  10(14)11539-11545.
  8. Chen Z, Liao P, Zhang F, et al. Centrifugal micro-channel array droplet generation for highly parallel digital PCR. Lab Chip 2017;17(2):235-40. doi: 10.1039/c6lc01305h
  9. Li S, Chen T, Wang Y, et al. Conjugated Polymer with Intrinsic Alkyne Units for Synergistically Enhanced Raman Imaging in Living Cells. Angew Chem Int Ed Engl 2017;56(43):13455-58. doi: 10.1002/anie.201707042
  10. Liao P, Huang Y. Digital PCR: Endless Frontier of ‘Divide and Conquer’. Micromachines 2017;8(8):231.
  11. Chen X, Huang Y. Editorial overview: Molecular imaging for seeing chemistry in biology. Curr Opin Chem Biol 2017;39:iv-v. doi: 10.1016/j.cbpa.2017.06.016
  12. Chen Z, Zhou W, Qiao S, et al. Highly accurate fluorogenic DNA sequencing with information theory-based error correction. Nat Biotechnol 2017;35(12):1170-78. doi: 10.1038/nbt.3982
  13. Chen T, Huang Y. Label-Free Transient Absorption Microscopy for Red Blood Cell Flow Velocity Measurement in Vivo. Anal Chem 2017;89(19):10120-23. doi: 10.1021/acs.analchem.7b01952
  14. Zheng C, Zhang X, Li C, et al. Microfluidic Device for Studying Controllable Hydrodynamic Flow Induced Cellular Responses. Anal Chem 2017;89(6):3710-15. doi: 10.1021/acs.analchem.7b00013
  15. Yang L, Ma Z, Cao C, et al. MR-seq: measuring a single cell’s transcriptome repeatedly by RNA-seq. Science Bulletin 2017;62(6):391-98. doi:
  16. Wu AR, Wang J, Streets AM, et al. Single-Cell Transcriptional Analysis. Annu Rev Anal Chem (Palo Alto Calif) 2017;10(1):439-62. doi: 10.1146/annurev-anchem-061516-045228
  17. Li C, Yu Z, Fu Y, et al. Single-Cell-Based Platform for Copy Number Variation Profiling through Digital Counting of Amplified Genomic DNA Fragments. ACS Applied Materials & Interfaces 2017;9(16):13958-64. doi: 10.1021/acsami.7b03146
  18. Yujiao Dang,# Liying Yan,# Boqiang Hu,# Xiaoying Fan, Yixin Ren, Rong Li, Ying Lian, Jie Yan, Qingqing Li, Yan Zhang, Min Li, Xiulian Ren, Jin Huang, Yuqi Wu, Ping Liu, Lu Wen, Chen Zhang, Yanyi Huang,* Fuchou Tang,* Jie Qiao* .Tracing the expression of circular RNAs in human pre-implantation embryos. Genome Biology 17 130 (2016) .doi: 10.1186/s13059-016-0991-3 (2016) .doi: 10.1021/acs.analchem.6b00862
  19. Yusi Fu, Chunmei Li, Sijia Lu, Wenxiong Zhou, Fuchou Tang, X. Sunney Xie,* Yanyi Huang*. Uniform and accurate single-cell sequencing based on emulsion whole-genome amplification. Proceedings of the National Academy of Sciences of the United States of America 112 (38), 11923-11928 (2015) .doi: 10.1073/pnas.1513988112
  20. Xiaoying Fan,# Xiannian Zhang,# Xinglong Wu, Hongshan Guo, Yuqiong Hu, Fuchou Tang,* Yanyi Huang*. Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos. Genome Biology 16 148 (2015) .doi: 10.1186/s13059-015-0706-1
  21. Jie Shen,# Dongqing Jiang,# Yusi Fu,# Xinglong Wu,# Hongshan Guo, Binxiao Feng, Yuhong Pang, Aaron M. Streets, Fuchou Tang,* Yanyi Huang*. H3K4me3 epigenomic landscape derived from ChIP-Seq of 1,000 mouse early embryonic cells. Cell Research 25 (1), 143-147 (2015) .doi: 10.1038/cr.2014.119
  22. Aaron M. Streets, Xiannian Zhang, Chen Cao, Yuhong Pang, Xinglong Wu, Liang Xiong, Lu Yang, Yusi Fu, Liang Zhao,* Fuchou Tang,* Yanyi Huang.* Microfluidic single-cell whole transcriptome sequencing. PNAS 2014, published online. doi: 10.1073/pnas.1402030111
  23. Senlian Hong, Tao Chen, Yuntao Zhu, Ang Li, Yanyi Huang,* Xing Chen.* Live-cell stimulated Raman scattering imaging of alkyne-tagged biomolecules. Angew. Chem. Int. Ed. 2014, published online. doi: 10.1002/anie.201400328
  24. Aaron M. Streets, Yanyi Huang.* Chip in a lab: microfluidics for next generation life science research. Biomicrofluidics 2013, 7(1), 011302.
  25. Chunhong Zheng, Zhilong Yu, Ying Zhou, Louis Tao, Yuhong Pang, Tao Chen, Xiannian Zhang, Haiwei Qiu, Hongwei Zhou, Zitian Chen, Yanyi Huang.* Live cell imaging analysis of the epigenetic regulation of the human endothelial cell migration at single cell resolution. Lab on a Chip 2012, 12(17), 3063-3072.
  26. Zhilong Yu, Tao Chen, Xiannian Zhang, Dan Fu, Xin Liao, Jie Shen, Xinxing Liu, Bo Zhang, X. Sunney Xie, Xiaodong Su, Junyuan Chen,* Yanyi Huang.* Label-free chemical imaging in vivo: three-dimensional non-invasive microscopic observation of amphioxus notochord through stimulated Raman scattering (SRS). Chemical Science 2012, 3(8), 2646-2654.
  27. Chunhong Zheng, Jingwen Wang, Yuhong Pang, Jianbin Wang, Wenbin Li, Zigang Ge,* Yanyi Huang.* High-throughput immunoassay through in-channel microfluidic patterning. Lab on a Chip 2012, 12(14), 2487-2490. (featured as inside front cover)
  28. Yongfan Men, Yusi Fu, Zitian Chen, Peter A. Sims, William J. Greenleaf, Yanyi Huang.* Digital polymerase chain reaction in an array of femtoliter PDMS microreactors. Analytical Chemistry 2012, 84(10), 4262-4266.
  29. Wentao Li, Tao Chen, Zitian Chen, Peng Fei, Zhilong Yu, Yuhong Pang, Yanyi Huang.* Squeeze-chip: A finger controlled microfluidic flow network device and its application to biochemical assays. Lab on a Chip 2012, 12(9), 1587-1590. (featured as inside front cover)
  30. Peng Fei, Zhilong Yu, Xu Wang, Peter J. Lu, Yusi Fu, Zi He, Jingwei Xiong, Yanyi Huang.* High dynamic range optical projection tomography (HDR-OPT). Optics Express 2012, 20(8), 8824-8836.
  31. Ying Zhou, Yuhong Pang, Yanyi Huang.* Openly Accessible Microfluidic Liquid Handlers for Automated High-throughput Nanoliter Cell Culture. Analytical Chemistry 2012, 84(5), 2576-2584.
  32. Chunhong Zheng, Liang Zhao, Gui’e Chen, Ying Zhou, Yuhong Pang, Yanyi Huang.* Quantitative Study of the Dynamic Tumor-endothelial Cell Interactions through an Integrated Microfluidic Coculture System. Analytical Chemistry 2012, 84(4), 2088-2093.
  33. Jie Shen, Ying Zhou, Tu Lu, Junya Peng, Zhixiang Lin, Lei Huang, Yuhong Pang, Li Yu, Yanyi Huang.* An Integrated Chip for Immunofluorescence and Its Application to Analyze Lysosomal Storage Disorders. Lab on a Chip 2012, 12, 317-324.
  34. Zuoyan Han, Wentao Li, Yanyi Huang, Bo Zheng. Measuring rapid enzymatic kinetics by electrochemical method in droplet-based microfluidic devices with pneumatic valves. Analytical Chemistry 2009, 81(14), 5840-5845.
  35. Jianbin Wang, Ying Zhou, Haiwei Qiu, Huang Huang, Changhong Sun, Jianzhong Xi, Yanyi Huang.* A Chip-to-chip nanoliter microfluidic dispenser. Lab on a Chip 2009, 9(13), 1831-1835.
  36. Yuanzi Wu, Yanyi Huang, Hongwei Ma. A facial method for permanent and functional surface modification of poly(dimethylsiloxane). Journal of the American Chemical Society 2007, 129(23), 7226-7227.
  37. Yanyi Huang, Piero Castrataro, Cheng-Chung Lee, Stephen R. Quake. Solvent resistant microfluidic DNA synthesizer. Lab on a Chip 2007, 7(1), 24-26.
  38. Wei Liang, Lan Yang, Joyce K. S. Poon, Yanyi Huang,* Amnon Yariv, Kerry Vahala. Transmission characteristics of a Fabry-Perot etalon-microtoroid resonator coupled system. Optics Letters 2006, 31(4), 510-512.
  39. Wei Liang, Yanyi Huang,* Yong Xu, Reginald K. Lee, Amnon Yariv. Highly-sensitive fiber Bragg grating refractive index sensors. Applied Physics Letters 2005, 86(15), 151122.
  40. Yanyi Huang, Yong Xu, Amnon Yariv. Fabrication of functional microstructured optical fibers through a selective filling technique. Applied Physics Letters 2004, 85(22), 5182-5184.
  41. Yanyi Huang,* George T. Paloczi, Joyce K. S. Poon, Amnon Yariv. Bottom-up softlithographic fabrication of three-dimensional multilayer polymer integrated optical microdevices. Applied Physics Letters 2004, 85(15), 3005-3007.
  42. Yanyi Huang,* George T. Paloczi, Amnon Yariv, Cheng Zhang, Larry R. Dalton. Fabrication and Replication of Polymer Integrated Optical Devices Using Electron-Beam Lithography and Soft Lithography. Journal of Physical Chemistry B 2004, 108(25), 8606-8613.
  43. Yanyi Huang,* George T. Paloczi, Joyce K. S. Poon, Amnon Yariv. Demonstration of flexible freestanding all-polymer integrated optical ring resonator devices. Advanced Materials 2004, 16(1), 44-48.