2015-Present, Peking Unviersity-Tsinghua University Center for Life Sciences, Principle Investigator

2010-Present, BIOPIC, College of Life Sciences, Peking University, Principle Investigator

2004-2010, Gurdon Institute, University of Cambridge, Postdoctoral Research Associate

2003, Ph. D., Peking University, Biology

1998, B. S., Peking University, Biology

Germline cells are the cells critical for transmitting genomic information from generation to generation to keep a multicellular organism species immortal. Germline cells include totipotent zygotes & early cleavage stage embryos, pluripotent inner cell mass (ICM) & epiblasts (EPI), primordial germ cells (PGCs), fetal germ cells (FGCs), female germ cells during oogenesis, male germ cells during spermatogenesis, mature oocytes and mature sperm. During germline development, all of the epigenetic changes need to be reset back (reprogramming) at the next generation. So the epigenetic regulations in germline cells are most complex and most accurate in multicellular organisms. Dissecting the molecular mechanisms of germline development is important for understanding human development, and for its application of precision medicine in order to cure related human diseases. Our lab focuses on the epigenetic mechanisms of human germline development as well as tumorigenesis. Through this, we want to understand the epigenetic regulation of human germline development and the global epigenetic programming & reprogramming during this process. We use single-cell sequencing technologies (single-cell genome, transcriptome, DNA methylome, chromatin accessibility, 3D genome, as well as multi-omics sequencing technologies), genome editing, embryo micromanipulation, derivation and culture of organoids, directed differentiation of human embryonic stem cells to analyze the epigenetic basis of the gene regulation network during human germline development at single-cell and single-base resolution. These works may lead to better diagnosis and treatment of related infertility, sterility, and tumors.

2021,The Xplorer Prize

2017, Bayer Investigator Award

2016, NSFC, Distinguished Young Investigator Award

2015, Guxiaocheng Lecture Award, Ray Wu Fouddation

2015, "Accurate Deduction of Maternal Genome Information" Listed as Top 10 China’s Scientific Advances in 2014

2013, NSFC, Excellent Young Investigator Award

1. Li M, Jiang Z, Xu X, Wu X, Liu Y, Chen K, Liao Y, Li W, Wang X, Guo Y, Zhang B, Wen L*, Kee K*, Tang Fuchou* (2025) Chromatin accessibility landscape of mouse early embryos revealed by single-cell NanoATAC-seq2. Science. 387: eadp4319
2. Li Q, Guo Y, Wu Z, Xu X, Jiang Z, Qi S, Liu Z, Wen L, Tang Fuchou* (2024) scNanoSeq-CUT&Tag: a single-cell long-read CUT&Tag sequencing method for efficient chromatin modification profiling within individual cells. Nature Methods. 21: 2044-2057
3. Liu Z, Hu Y, Xie H, Chen K, Wen L, Fu W, Zhou X*, Tang Fuchou* (2024) Single-cell chromatin accessibility analysis reveals the epigenetic basis and signature transcription factors for the molecular subtypes of colorectal cancers. Cancer Discovery. 14: 1082-1105
4. Bai X, Chen Z, Chen K, Wu Z, Wang R, Liu J, Chang L, Wen L, Tang Fuchou* (2024) Simultaneous de novo calling and phasing of genetic variants at chromosome-scale using NanoStrand-seq. Cell Discovery. 10: 74
5. Li W, Lu J, Lu P, Gao Y, Bai Y, Chen K, Su X, Li M, Liu J, Chen Y, Wen L, Tang Fuchou* (2023) scNanoHi-C: a single-cell long-read concatemer sequencing method to reveal high-order chromatin structures within individual cells. Nature Methods. 20: 1493-1505
6. Lin J, Xue X, Wang Y, Zhou Y, ..., Liu M, Wen L, Tang Fuchou* (2023) scNanoCOOL-seq: a long-read single-cell sequencing method for multi-omics profiling within individual cells. Cell Research. 33: 879-882
7. Hu Y, Jiang Z, Chen K, Zhou Z, Zhou X, Wang Y, Yang J, Zhang B, Wen L, Tang Fuchou* (2023) scNanoATAC-seq: a long-read single-cell ATAC sequencing method to detect chromatin accessibility and genetic variants simultaneously within an individual cell. Cell Research. 33: 83-86
8. Xie H, Li W, Hu Y, Yang C, Lu J, Guo Y, Wen L, Tang Fuchou* (2022) De novo assembly of human genome at single-cell levels. Nucleic Acids Research. 50: 7479-7492
9. Fan X, Yang C, Li W, Bai X, Zhou X, Xie H, Wen L, Tang Fuchou* (2021) SMOOTH-seq: single-cell genome sequencing of human cells on a third-generation sequencing platform. Genome Biology. 22: 195
10. Zhou Y, Bian S, ..., Fu W*, Tang Fuchou* (2020) Single-Cell Multiomics Sequencing Reveals Prevalent Genomic Alterations in Tumor Stromal Cells of Human Colorectal Cancer. Cancer Cell. 38: 818-828
11. Zhou F, Wang R, Yuan P, Ren Y, ..., Lian Y, Li J, Wen L, Yan L, Qiao J*, Tang Fuchou* (2019) Reconstituting the transcriptome and DNA methylome landscapes of human implantation. Nature. 572: 660-664
12. Zhu P, Guo H, Ren Y, ..., Wen L, Yan L*, Qiao J*, Tang Fuchou* (2018) Single-cell DNA methylome sequencing of human preimplantation embryos. Nature Genetics. 50: 12-19
13. Bian S, Hou Y, Zhou X, Li X, Yong J, ..., Zhu P, Xiu D, Yan L, Wen L, Qiao J*, Tang Fuchou*, Fu W* (2018) Single-cell multiomics sequencing and analyses of human colorectal cancer. Science. 362: 1060-1063
14. Guo F, Yan L, Guo H, Li L, Hu B, Zhao Y, Yong J, Hu Y, Wang X, Wei Y, Wang W, Li R, ......, Wen L, Gao Y, Tang Fuchou*, Qiao J* (2015) The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells. Cell. 161, 1437-1452
15. Guo H, Zhu P, Yan L, Li R, Hu B, Lian Y, Yan J, Ren X, Lin S, Li J, Jin X, Shi X, Liu P, Wang X, Wang W, Wei Y, Li X, Guo F, Wu X, Fan X, Yong J, Wen L, Xie SX, Tang Fuchou*, Qiao J* (2014) Nature. 511: 606-610
16. Hou Y, Fan W, Yan L, Li R, Lian Y, Huang J, Li J, Xu L, Tang Fuchou*, Xie XS*, Qiao J* (2013) Genome analyses of single human oocytes. Cell. 155:1492-1506