Add:Rm.203, Biomedical Pioneering Innovation Center, Integrated Science Research Center No.2,Peking University
Mailing Address:BIOPIC, Integrated Science Research Center No.2, Peking University, Yiheyuan Rd., No. 5, Haidian District, Beijing, 100871

Lab Web:

Dong Xing

2019-Present, Assistance ProfessorPeking University

2012-2019, Postdoctoral Fellow, Harvard University

Research Interests

DNA is the major genetic material. In eukaryotic cells, genomic DNA, which can be as long as several meters, are folded inside a nucleus of a few micrometers in diameter. Despite this tight packing, important genomic processes, such as transcription and DNA replication, remain functional. The key to understand how genetic information are expressed and transmitted relies on the study of the genome. We are interested in developing methods to probe both the structure and function of the genome in single cells, including accurate amplification and sequencing of the single-cell whole genome, reconstruction of the three-dimensional genome structure and detection of chromatin accessible regions, positioning of nucleosomes and DNA-protein interactions. By leveraging the power of these cutting-edge technologies, we focus on the study of basic biological questions, such as genome stability, regulation of gene expression and the role of 3D genome organization.

Selected Publication
  1. Liu Z, Chen Y, Xia Q, Liu M, Xu H, Chi Y, Deng Y, Xing D. Linking genome structures to functions by simultaneous single-cell Hi-C and RNA-seq. Science. 2023 Jun 9;380(6649):1070-1076. doi: 10.1126/science.adg3797. Epub 2023 Jun 8. PMID: 37289875.
  2. L. Tan, D. Xing, N. Daley, X. S. Xie, Three-dimensional genome structures of single sensory neurons in mouse visual and olfactory systems. Nat Struct Mol Biol 26, 297-307 (2019).
  3. L. Tan, D. Xing, C. H. Chang, H. Li, X. S. Xie, Three-dimensional genome structures of single diploid human cells. Science 361, 924-928 (2018).
  4. C. Chen, D. Xing, L. Tan, H. Li, G. Zhou, L. Huang, X. S. Xie, Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI). Science 356, 189-194 (2017).
  5. Z. Liu, D. Xing, Q. P. Su, Y. Zhu, J. Zhang, X. Kong, B. Xue, S. Wang, H. Sun, Y. Tao, Y. Sun, Super-resolution imaging and tracking of protein-protein interactions in sub-diffraction cellular space. Nat Commun 5, 4443 (2014).
  6. S. Kim, E. Brostromer, D. Xing, J. Jin, S. Chong, H. Ge, S. Wang, C. Gu, L. Yang, Y. Q. Gao, X. D. Su, Y. Sun, X. S. Xie, Probing allostery through DNA. Science 339, 816-819 (2013).