北京大学生物医学前沿创新中心

联系方式

E-mail：sunneyxie@pku.edu.cn
办公电话：+86-010-62744061
办公地点：北京大学综合科研2号楼105室
邮件地址：北京市海淀区颐和园路5号综合科研2号楼107室，100871

研究组主页：http://sunneyxielab.org/

谢晓亮

BIOPIC 研究员；ICG 主任、研究员

个人履历

2020-现在，昌平实验室主任

2019.11-现在，北京大学理学部主任

2018.7-现在，北京大学李兆基讲席教授

2016.8-现在，北京未来基因诊断高精尖创新中心(ICG)，主任

2010-现在，北京大学生物医学前沿创新中心(BIOPIC)，研究员

2010-2022.3，北京大学生物医学前沿创新中心(BIOPIC)，主任

2009-现在，北京大学生命科学学院教授

2009-2018，哈佛大学化学与化学生物学系，Mallinckrodt讲席教授

1999-2009，哈佛大学化学与化学生物学系，终身教授

1992-1998，美国太平洋西北国家实验室(PNNL)，环境分子科学实验室，Senior Research Scientist to Chief Scientist，是PNNL1965年成立以来第一位来自中国的科学家

1990-1992，美国芝加哥大学博士后研究

1990，理学博士，美国加州大学圣地亚哥分校

1984，理学学士，北京大学

主要研究方向

谢晓亮课题组目前的研究方向包含：

(1)单细胞测序技术的开发。如单细胞基因组测序、转录组测序、甲基化测序、染色质三维结果解析、染色质开放性检测以及单细胞多组学等； (2)单细胞测序技术在基因表达调控中的应用。如转录因子调控基因表达机理、早期胚胎发育过程中的表观修饰动态变化、表观遗传如何协调影响基因调控网络构建等； (3)单细胞测序技术在医学转化上的应用。如癌症早期筛查诊断、无创产前遗传筛查、高通量抗体药物筛选等。

谢晓亮教授是单分子酶学的创始人、单分子生物物理化学的奠基人之一、相干拉曼散射显微成像技术和单细胞基因组学的开拓者。近年来，谢晓亮在基因组学、特别是单细胞测序技术方面取得了一系列重要成果，为生命科学和医学的深入研究应用提供了重要的手段。

获奖及荣誉

2025，北京自然科学一等奖

2024，腾冲科学大奖

2022，突出贡献中关村奖

2017，中国科学院院士

2017，求是杰出科学家奖

2016，华人生物学家协会吴瑞奖

2015，中国生物物理学会贝时璋生物物理奖

2015，美国Albany 生物医学奖

2015，美国化学会Peter Debye 物理化学奖

2014，美国光学会会士

2014，德国洪堡学者

2013，美国NIH Director's Pioneer Award

2013，美国光学学会及应用光谱学会Ellis R. Lippincott奖

2012，美国化学会Rochester分部Harrison Howe奖

2012，美国微生物学会会士

2012，美国生物物理学会Founders奖

2010，中国化学会荣誉会士

2009，美国能源部劳伦斯奖，化学类

2008，美国物理学会会士

2008，Berthold Leibinger Zukunftspreis激光奖

2007，Willis E.Lamb激光科学与量子光学奖

2006，美国科学促进会(AAAS)会士

2006，美国生物物理学会会士

2004，美国NIH Director's Pioneer Award

2003，以色列Raymond and Beverly Sackler 物理科学奖

1996，Coblentz协会，Coblentz奖

代表性论文及论著

Single Cell Genomics

Wu, H.; Zhang, J.; Tan, L.; Xie, X. Single-cell Micro-C profiles 3D genome structures at high resolution and characterizes multi-enhancer hubs. Nat Genet. 2025, 57, 1777–1786. 10.1038/s41588-025-02247-6
He, R.; Dong, W.; Wang, Z.; Xie, C.; Gao, L.; Ma, W.; Shen, K.; Li, D.; Pang, Y.; Jian, F.; Zhang, J.; Yuan, Y.; Wang, X.; Zhang, Z.; Zhen, Y.; Liu, S.; Luo, C.; Chai, X.; Ren, J.; Zhu, Z.; Xie, X. Genome-wide single-cell and single-molecule footprinting of transcription factors with deaminase. PNAS. 2024, 121(52), e2423270121. 10.1073/pnas.242327012
Chapman, A.; Lee, D.; Cai, W.; Ma, W.; Li, X.; Sun, W.; Xie, X. Correlated gene modules uncovered by high-precision single-cell transcriptomics. PNAS. 2022, 119(51), e2206938119. 10.1073/pnas.2206938119
Tan, L.; Ma, W.; Wu, H.; Sun, W.; Zheng, Y.; Xing, D.; Chen, R.; Li, X.; Daley, N.; Deisseroth, K.; Xie, X. Changes in genome architecture and transcriptional dynamics progress independently of sensory experience during post-natal brain development. Cell. 2021, 184(3), 741-758. 10.1016/j.cell.2020.12.032
Tan, L.; Xing, D.; Chang, C.; Li, H.; Xie, X. Three-dimensional genome structures of single diploid human cells. Science. 2018, 361, 924-928. 10.1126/science.aat5641
Chen, C.; Xing, D.; Tan, L.; Li, H.; Zhou, G.; Huang, L.; Xie, X. Single-cell whole-genome analyses by Linear Amplification via Transposon Insertion (LIANTI). Science. 2017, 356, 189-194. 10.1126/science.aak9787
Yan, L.; Huang, L.; Xu, L.; Huang, J.; Ma, F.; Zhu, X.; Tang, Y.; Liu, M.; Lian, Y.; Liu, P.; Li, R.; Lu, S.; Tang, F.; Qiao, J.; Xie, X. Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with linkage analyses. PNAS. 2015, 112(52), 15964-15969. 10.1073/pnas.1523297113
Hou, Y.; Fan, W.; Yan, L.; Li, R.; Lian, Y.; Huang, J.; Li, J.; Xu, L.; Tang, F.; Xie, X.; Qiao, J. Genome Analyses of Single Human Oocytes. Cell. 2013, 155(7), 1492-1506. 10.1016/j.cell.2013.11.040
Ni, X.; Zhuo, M.; Su, Z.; Duan, J.; Gao, Y.; Wang, Z.; Zong, C.; Bai, H.; Chapman, A.; Zhao, J.; Xu, L.; An, T.; Ma, Q.; Wang, Y.; Wu, M.; Sun, Y.; Wang, S.; Li, Z.; Yang, X.; Yong, J.; Su, X.; Lu, Y.; Bai, F.; Xie, X.; Wang, J. Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients. PNAS. 2013, 110(52), 21083-21088. 10.1073/pnas.1320659110
Zong, C.; Lu, S.; Chapman, A.; Xie, X. Genome-Wide Detection of Single-Nucleotide and Copy-Number Variations of a Single Human Cell. Science. 2012, 338(6114), 1622-1626. 10.1126/science.1229164
Lu, S.; Zong, C.; Fan, W.; Yang, M.; Li, J.; Chapman, A.; Zhu, P.; Hu, X.; Xu, L.; Yan, L.; Bai, F.; Qiao, J.; Tang, F.; Li, R.; Xie, X. Probing Meiotic Recombination and Aneuploidy of Single Sperm Cells by Whole-Genome Sequencing. Science. 2012, 338(6114), 1627-1630. 10.1126/science.1229112
Shiroguchi, K.; Jia, T.; Sims, P.; Xie, X. Digital RNA sequencing minimizes sequence-dependent bias and amplification noise with optimized single-molecule barcodes. PNAS. 2012, 109(4), 1347-1352. 10.1073/pnas.1118018109
Sims, P.; Greenleaf, W.; Duan, H.; Xie, X. Fluorogenic DNA sequencing in PDMS microreactors. Nat Meth. 2011, 8(7), 575-580. 10.1038/nmeth.1629

Coherent Raman Microscopy

Ji, M.; Orringer, D.; Freudiger, C.; Ramkissoon, S.; Liu, X.; Lau, D.; Golby, A.; Norton, I.; Hayashi, M.; Agar, N.; Young, G.; Spino, C.; Santagata, S.; Camelo-Piragua, S.; Ligon, K.; Sagher, O.; Xie, X. Rapid, Label-Free Detection of Brain Tumors with Stimulated Raman Scattering Microscopy. Science Translational Medicine. 2013, 5(201), 201ra119-201ra119. 10.1126/scitranslmed.3005954
Wang, M.; Min, W.; Freudiger, C.; Ruvkun, G.; Xie, X. RNAi screening for fat regulatory genes with SRS microscopy. Nat Meth. 2011, 8(2), 135-138. 10.1038/nmeth.1556
Saar, B.; Freudiger, C.; Reichman, J.; Stanley, C.; Holtom, G.; Xie, X. Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering. Science. 2010, 330(6009), 1368-1370. 10.1126/science.1197236
Freudiger, C.; Min, W.; Saar, B.; Lu, S.; Holtom, G.; He, C.; Tsai, J.; Kang, J.; Xie, X. Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy. Science. 2008, 322(5909), 1857-1861. 10.1126/science.1165758
Evans, C.; Potma, E.; Puoris'haag, M.; Cote, D.; Lin, C.; Xie, X. Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy. PNAS. 2005, 102(46), 16807-16812. 10.1073/pnas.0508282102
Zumbusch, A.; Holtom, C.; Xie, X. Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering. Physical Review Letters. 1999, 82, 4142-4145.

Gene Expression and Regulation - Live Cell Single Molecule Studies

Taniguchi, Y.; Choi, P.; Li, G.; Chen, H.; Babu, M.; Hearn, J.; Emili, A.; Xie, X. Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells. Science. 2010, 329(5991), 533-538. 10.1126/science.1188308
Choi, P.; Cai, L.; Frieda, K.; Xie, X. A Stochastic Single-Molecule Event Triggers Phenotype Switching of a Bacterial Cell. Science. 2008, 322(5900), 442-446. 10.1126/science.1161427
Elf, J.; Li, G.; Xie, X. Probing Transcription Factor Dynamics at the Single-Molecule Level in a Living Cell. Science. 2007, 316(5828), 1191-1194. 10.1126/science.1141967
Yu, J.; Xiao, J.; Lao, K.; Xie, X. Probing Gene Expression in Live Cells, One Protein Molecule at a Time. Science. 2006, 311(5767), 1600-1603. 10.1126/science.1119623
Cai, L.; Friedman, N.; Xie, X. Stochastic protein expression in individual cells at the single molecule level. Nature. 2006, 440(7082), 358-362. 10.1038/nature04599

Single Molecule Enzymology

Chong, S.; Chen, C.; Ge, H.; Xie, X. Mechanism of Transcriptional Bursting in Bacteria. Cell. 2014, 158(2), 314-326. 10.1016/j.cell.2014.05.038
Kim, S.; Brostromer, E.; Xing, D.; Jin, J.; Chong, S.; Ge, H.; Wang, S.; Gu, C.; Yang, L.; Gao, Y.; Su, X.; Sun, Y.; Xie, X. Probing Allostery Through DNA. Science. 2013, 339(6121), 816-819. 10.1126/science.1229223
English, B.; Min, W.; van Oijen, A.; Lee, K.; Luo, G.; Sun, H.; Cherayil, B.; Kou, S.; Xie, X. Ever-fluctuating single enzyme molecules: Michaelis-Menten equation revisited. Nat Chem Biol. 2006, 2(2), 87-94. 10.1038/nchembio759
Yang, H.; Luo, G.; Karnchanaphanurach, P.; Louie, T.; Rech, I.; Cova, S.; Xun, L.; Xie, X. Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer. Science. 2003, 302(5643), 262-266. 10.1126/science.1086911
Lu, H.; Xun, L.; Xie, X. Single Molecule Enzymatic Dynamics. Science. 1998, 282, 1877.

Single Molecule Spectroscopy and Imaging

Chong, S.; Min, W.; Xie, X. Ground-State Depletion Microscopy: Detection Sensitivity of Single-Molecule Optical Absorption at Room Temperature. The Journal of Physical Chemistry Letters. 2010, 1(23), 3316-3322. 10.1021/jz1014289
Min, W.; Lu, S.; Chong, S.; Roy, R.; Holtom, G.; Xie, X. Imaging chromophores with undetectable fluorescence by stimulated emission microscopy. Nature. 2009, 461(7267), 1105-1109. 10.1038/nature08438
Sánchez, E.; Novotny, L.; Xie, X. Near-field Fluorescence Microscopy Based on Two-Photon Excitation With Metal Tips. Phys. Rev. Lett. 1999, 82, 4014.
Lu, H.; Xie, X. Single-Molecule Spectral Fluctuations at Room Temperature. Nature. 1997, 385, 143.
Sánchez, E.; Novotny, L.; Holtom, G.; Xie, X. Room-Temperature Fluorescence Imaging and Spectroscopy of Single Molecules by Two-Photon Excitation. J. Phys. Chem. A. 1997, 101, 7019.
Xie, X.; Dunn, R. Probing Single Molecule Dynamics. Science. 1994, 265, 361.

COVID-19

Cao, Y.; Jian, F.; Wang, J.; Yu, Y.; Song, W.; Yisimayi, A.; Wang, J.; An, R.; Chen, X.; Zhang, N.; Wang, Y.; Wang, P.; Zhao, L.; Sun, H.; Yu, L.; Yang, S.; Xiao, T.; Gu, Q.; Shao, F.; Hao, X.; Xu, Y.; Jin, R.; Shen, Z.; Wang, Y.; Xie, X. Imprinted SARS-CoV-2 humoral immunity induces convergent Omicron RBD evolution. Nature. 2023, 614, 521-529. 10.1038/s41586-022-05644-7
Cao, Y.; Jian, F.; Zhang, Z.; Yisimayi, A.; Hao, X.; Bao, L.; Yuan, F.; Yu, Y.; Du, S.; Wang, J.; Xiao, T.; Song, W.; Zhang, Y.; Liu, P.; An, R.; Wang, P.; Wang, Y.; Yang, S.; Niu, X.; Zhang, Y.; Gu, Q.; Shao, F.; Hu, Y.; Yin, W.; Zheng, A.; Wang, Y.; Qin, C.; Jin, R.; Xiao, J.; Xie, X. Rational identification of potent and broad sarbecovirus-neutralizing antibody cocktails from SARS convalescents. Cell Reports. 2022, 41(12), 111845. 10.1016/j.celrep.2022.111845
Cao, Y.; Song, W.; Wang, L.; Liu, P.; Yue, C.; Jian, F.; Yu, Y.; Wang, Y.; Yisimayi, A.; Wang, P.; Wang, Y.; Zhu, Q.; Deng, J.; Fu, W.; Yu, L.; Zhang, N.; Wang, J.; Xiao, T.; An, R.; Wang, J.; Liu, L.; Yang, S.; Niu, X.; Gu, Q.; Shao, F.; Hao, X.; Meng, B.; Gupta, R.; Jin, R.; Wang, Y.; Xie, X.; Wang, X. Characterization of the enhanced infectivity and antibody evasion of Omicron BA.2.75. Cell Host & Microbe. 2022, 30(11), 1527-1539. 10.1016/j.chom.2022.09.018
Cao, Y.; Yisimayi, A.; Jian, F.; Song, W.; Xiao, T.; Wang, L.; Du, S.; Wang, J.; Wang, J.; Li, Q.; Chen, X.; Yu, Y.; Wang, P.; Zhang, Z.; Liu, P.; An, R.; Hao, X.; Wang, Y.; Wang, J.; Feng, R.; Sun, H.; Zhao, L.; Zhang, W.; Zhao, D.; Zheng, J.; Yu, L.; Li, C.; Zhang, N.; Wang, R.; Niu, X.; Yang, S.; Song, X.; Chai, Y.; He, Y.; Shi, Y.; Zheng, L.; Li, Z.; Gu, Q.; Shao, F.; Huang, W.; Jin, R.; Shen, Z.; Wang, Y.; Wang, X.; Xiao, J.; Xie, X. BA.2.12.1, BA.4 and BA.5 escape antibodies elicited by Omicron infection. Nature. 2022, 608, 593-602. 10.1038/s41586-022-04980-y
Cao, Y.; Wang, J.; Jian, F.; Xiao, T.; Song, W.; Yasimayi, A.; Huang, W.; Li, Q.; Wang, P.; An, R.; Wang, J.; Wang, Y.; Niu, X.; Yang, S.; Liang, H.; Sun, H.; Li, T.; Yu, Y.; Cui, Q.; Liu, S.; Yang, X.; Du, S.; Zhang, Z.; Hao, X.; Shao, F.; Jin, R.; Wang, X.; Xiao, J.; Wang, Y.; Xie, X. Omicron escapes the majority of existing SARS-CoV-2 neutralizing antibodies. Nature. 2021, 602, 657-663. 10.1038/s41586-021-04385-3
Cao, Y.; Yisimayi, A.; Bai, Y.; Huang, W.; Li, X.; Zhang, Z.; Yuan, T.; An, R.; Wang, J.; Xiao, T.; Du, S.; Ma, W.; Song, L.; Li, Y.; Li, X.; Song, W.; Wu, J.; Liu, S.; Li, X.; Zhang, Y.; Su, B.; Guo, X.; Wei, Y.; Gao, C.; Zhang, N.; Zhang, Y.; Dou, Y.; Xu, X.; Shi, R.; Lu, B.; Jin, R.; Ma, Y.; Qin, C.; Wang, Y.; Feng, Y.; Xiao, J.; Xie, X. Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines. Cell Research. 2021, 31(7), 732-741. 10.1038/s41422-021-00514-9
Du, S.; Cao, Y.; Zhu, Q.; Yu, P.; Qi, F.; Wang, G.; Du, X.; Bao, L.; Deng, W.; Zhu, H.; Liu, J.; Nie, J.; Zheng, Y.; Liang, H.; Liu, R.; Gong, S.; Liu, J.; Xu, H.; Yisimayi, A.; Lv, Q.; Wang, B.; He, R.; Han, Y.; Zhao, W.; Bai, Y.; Qu, Y.; Gao, X.; Ji, C.; Wang, Q.; Gao, N.; Huang, W.; Wang, Y.; Xie, X.; Su, X.; Xiao, J.; Qin, C. Structurally resolved SARS-CoV-2 antibody shows high efficacy in severely infected hamsters and provides a potent cocktail pairing strategy. Cell. 2020, 183(4), 1013-1023.e13. 10.1016/j.cell.2020.09.035
Cao, Y.; Su, B.; Guo, X.; Sun, W.; Deng, Y.; Bao, L.; Zhu, Q.; Zhang, X.; Zheng, Y.; Geng, C.; Chai, X.; He, R.; Li, X.; Lv, Q.; Zhu, H.; Deng, W.; Xu, Y.; Wang, Y.; Qiao, L.; Tan, Y.; Song, L.; Wang, G.; Du, X.; Gao, N.; Liu, J.; Xiao, J.; Su, X.; Du, Z.; Feng, Y.; Qin, C.; Qin, C.; Jin, R.; Xie, X. Potent Neutralizing Antibodies against SARS-CoV-2 Identified by High-Throughput Single-Cell Sequencing of Convalescent Patients' B Cells. Cell. 2020, 182(1), 73-84.e16. 10.1016/j.cell.2020.05.025