报告题目：Novel cell types in the mouse cortex and hippocampus revealed by 

          single-cell RNA-seq

报告人：Prof. Sten Linnarsson

        Karolinska Institutet

时间：2014年12月8日（周一）上午10：00

地点：北京大学综合科研楼302会议室

联系人：黄岩谊（62744058）

摘要：The mammalian cerebral cortex supports cognitive functions such as sensorimotor integration, memory, and social behaviours. Its normal functioning relies on the accurate assembly of a diverse set of differentiated cell types, including neurons, glia and vasculature. However, currently there is no comprehensive catalog of cortical cell types. Here, we have used large-scale single-cell RNA-seq to classify cells in the mouse somatosensory cortex and hippocampal CA1 region. We found 47 molecularly distinct subclasses, comprising all known major cell types in the cortex. We identified numerous novel marker genes, which allowed alignment with known cell types, morphology and location. We found novel cell types including a novel layer I interneuron, a previously unrecognized type of perivascular microglia, and a distinct post-mitotic oligodendrocyte subtype. Across this diverse set of cell types, transcription factors formed a complex, layered regulatory code, suggesting a mechanism for the maintenance of adult cell type identity.