报告题目：Single cell transcriptional profiling by sequential FISH

报告人：Prof. Long Cai

        California Institute of Technology

时间：2014年12月15日（周一）上午10：30

地点：北京大学综合科研楼302会议室

联系人：黄岩谊（62744058）

摘要：We have recently demonstrated a technology using sequential hybridization and single molecule FISH to multiplex a large number of mRNA molecules directly in single cells in complex tissue samples.  We call this method seqFISH and it is conceptually akin to “sequencing” mRNAs directly in cells by FISH.  My lab is applying this technology to profile 100 or more genes directly in situ.  Using seqFISH, we will be able to understand the combinatorial expression patterns in single cells that would provide molecular identifier or “barcodes” for cell spatially heterogeneous samples.