11月1日（下周二）学术报告

报告人：东京大学应用化学系教授野地博行（Hiroyuki Noji）

Title: Recent advance of single-molecule biophysics of the ATP-driven rotary motor, F1-ATPase

Location: BIOPIC conference room

Time: 5pm, Tuesday ,Nov. 1st, 2011 

Abstract:
He will deliver recent advances made in single-molecule biophysical studies on F1-ATPase. F1-ATPase is the water soluble portion of the ATP synthase. When isolated, F1 acts as a rotary motor protein that rotates the inner subunit, gamma hydrolyzing ATP. The remarkable feature of this motor is that it can catalyze the reverse reaction; when the rotary shaft is mechanically reversed, F1 catalyzes ATP synthesis reaction against large chemical potential of ATP hydrolysis. Such a catalyst has not been found or invented in natural system or synthetic chemistry. To achieve the fine mechanochemical coupling feature, F1 must be able to modulate chemical equilibriums and rate constants of elementary reactions constituting ATP hydrolysis reaction in response to the external mechanical manipulation on the rotary shaft. To reveal this, they carried out single-moleule manipulation works to determine the ATP binding affinity and ATP hydrolysis equilibrium as the function of the rotary angle (Nature Chemical Bio. 2010 and in press). While the ATP binding state is largely stabilized upon rotation, the equilibrium constant of hydrolysis does not depend on the angle so much as binding process, suggesting that F1 generates much larger torque in ATP binding process than hydrolysis process. He also like to introduce our recent topic on cooperative power strokes in the isolated stator ring of F1-ATPase revealed with high speed atomic force microscopy (AFM). (Science 2011) This result suggests the intrinsic cooperativity of other hexameric ATPases such as RecA type proteins or AAA proteins.

Hiroyuki Noji

1. Research Area
- Molecular mechanisms of the energy conversion of the FoF1-ATP synthase
- Digital Counting of biomolecules using Million droplets Array

2. Research Highlights
- Direct observation of rotation of F1-ATPase by single molecule technique (Nature 1997)
- Development of femtoliter reaction chamber array for single molecule analysis (Nature Biotechnology 2005)
- Demonstration of high ATP synthesis efficiency (nearly100% chemomechanical coupling) of F1-ATPase by single molecule manipulation (Nature 2005)
- Development of FRET-based ATP sensor for intracellular ATP analysis (PNAS 2009)
- Million droplets array (Lab on a Chip 2010)
- Mechanochemistry of F1-ATPase (Nature Chemical Biology 2010 and to be appear in 2012)
- Intrisic cooperativity of torque generating unit in the stator ring of F1-ATPase (Science 2011)

3. Awards
- JSPS Prize, Japan Society for the Promotion of Science 2006
- Grand Prize, Amersham Pharmacia Biotech & Science Prize for Young Scientists 1998
- Tejima Prize for Doctoral Dissertation Award, Tejima Seiichi Commemorative Foundation 1999

4. International Symposium Organization　(Chair of organizing committee)
- 10th Sanken International Symposium on Nanoscience and Nanotechnology 2006, Osaka (Japan), 2006/9/19-20
- The 17th CDB Symposium “Towards Synthesis of Cell”, Kobe (Japan) 2008/10/14-15
- International Symposium of Post-Silicon Materials and Devices Research Alliance Project, Osaka (Japan), 2009/9/5-6
- International Symposium “Innovative Nanoscience of Supermolecular Motor Proteins working in Biomembranes”, Kyoto (Japan), 2009/9/7-9 

5. Other Contributions
- Project leader, “Nanobiotechnology for membrane proteins”, Bio-oriented Technology Research Advancement Institute (BRAIN), 2002-2007
- Project leader, “Innovative nanoscience of supramolecular motor proteins working in membrane”, Grant-in-Aid for Scientific Research on Priority Areas, The Ministry of Education, Culture, Sports, Science and Technology (MEXT), 2007-2011
- Member of peer-review committee, Grant-in-Aid for Scientific Research, MEXT, 2005-2008
- Adviser, “Structure and control of interfaces”, PRESTO program, Japan Science and Technology Agency (JST), 2006-present
- Board member, Biophysical Society of Japan, 2006-2007, 2010-present
- Board member, Protein Science Society of Japan, 2006-2007
- Vice chair, Biomicrosystems group, Sensors and Micromachines Society, The Institute of Electrical Engineering of Japan (IEEEJ), 2006-prsent
- Editorial Advisory Board, Protein Science, USA, 2009-present
- Committee member, Nanobio task force, nanotechnology?material science project, Council for Science and Technology, Cabinet office, Government of Japan, 2008-2009
- Project leader, Digital Counting device project, CREST, JST
- President, Japanese Society of Cell Synthesis Research, 2010-2011